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Microbial growth curve

Total questions: 22

Worksheet time: 53mins

Name
Class
Date
1.

Give any Two example of mild thermal processing ________&________(ALL CAPS - DIVIDE WITH COMMA NO SPACES)

(a)  

2.

Examples of Heat resistant enzymes ________&________(ALL CAPS DIVIDE BY COMMA NO SPACE)

(a)  

3.

Which describes the level of microbial destruction obtained by thermal treatment?

a)

F○ value

b)

Thermal death time

c)

D value

d)

Z value

4.

________ relates the resistance of an organism to differing temperatures.

a)

F value

b)

Thermal death time

c)

Z value

d)

D value

5.

_________ is defined as the time taken at a given temperature to eliminate at least 90% of the exposed microorganisms

a)

F value

b)

Thermal death time

c)

D value

d)

Z value

6.

which of the following is spore forming thermophilic anaerobe ?

a)

B.sterothermophilus

b)

C.thermosaccharolyticum

c)

C.sporogenes

d)

Desutfomotomaculum nigrificans

7.

which of the following blanching works in two stage process and results in higher efficiency?

a)

Blancher cooker

b)

Pipe blancher

c)

steam blancher

d)

Individual quick blanching

8.

which of the following statements are right, regarding Testing of the Effectiveness of Blanching

a)

Peroxidase enzyme is not in itself implicated in degradation

b)

guaiacol solution and hydrogen peroxide solution are added to detect the peroxidase

c)

Peroxidase is is relatively heat resistant and easily detected.

d)

observing the development of a Red colour indicating peroxidase activity

9.

(a)   is a naturally occurring enzyme in raw milk with a similar D value to heat-resistant pathogens and so is routinely used as an indicator of adequate pasteurisation.( ALL SPACE GIVE SPACE IF NESSESSARY)

10.

organisms that can survive exposure to relatively high temperatures but do not necessarily grow at these temperatures

a)

Thermophilic

b)

Mesophilic

c)

psychrophiles

d)

none of the above

11.

In Steam tunnel Pasteurizer, the temperatures in the heating zones may be controlled depending on the amount of _________

a)

water

b)

pH

c)

air

d)

none of these

12.

“Commercial sterility” - Identify the wrong statement

a)

Its the complete destruction of all the microorganisms in the product

b)

Most heat resistant pathogen is Clostridium botulinum.

c)

Most heat-resistant (non-pathogenic) spoilage microorganisms are Bacillus stearothermophilis and Clostridium thermosaccharolytom

d)

canning will come under UHT or aseptically processed sterilization

13.

Simple small autoclaves are ____________, Larger autoclaves are __________(HORIZONTAL,VERTICAL)

(a)  

14.

D value is also known as

a)

Decimal reduction time

b)

Thermal death time

c)

Decimal reduction point

d)

none

15.

“botulinum cook” time and temperature

a)

12 mins - 121

b)

4 min - 121

c)

0.21 mins - 121

d)

2.5 min - 121

16.

106 spores of Bacillus stearothermophillus are present we want it to reduce to 0.1 spores - how much log reduction cycle has to happen

a)

5D

b)

12D

c)

4D

d)

7D

17.

1000 Clostridium spores are present in the sample, we are giving 1010C to reducing those spore to 1- how much time needed ? ( calculate the D value - You already know the z value and D value for 121oC )

a)

21

b)

12

c)

42

d)

63

18.

A product is held at a temperature of 118°C for a period of 12 minutes and L value is 0.49min, How much time required if same product held at 1210C ? ( Hint- calculate F value using the values)

a)

6min

b)

4.9 mins

c)

4.6 mins

d)

5.9 mins

19.

The portion of the growth curve where rapid growth of bacteria is observed is known as ____________

a)

Lag phase

b)

Log phase

c)

Stationary phase

d)

Death phase

20.

In which of the following phase secondary metabolites are produced during growth?

a)

Lag phaseb

b)

Log/Exponential phase

c)

Stationary phased

d)

Death phase

21.

Which phase shows the reproduction rate equal to the equivalent death rate?

a)

Log phase

b)

Stationary phase

c)

Death phase

d)

Lag phase

22.

The average size of the cells in the exponential phase is ___________

a)

larger than the initial size

b)

smaller than the initial size

c)

equal to the initial size

d)

maybe smaller or larger than the initial size