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BIOTECHNOLOGY

Total questions: 10

Worksheet time: 5mins

Name
Class
Date
1.

What technique is used for separating DNA fragments?

a)

Gel electrophoresis

b)

Chromatography

c)

Transformation

d)

Transduction

2.

DNA fragments are

a)

negatively charged

b)

neutral

c)

either negatively or positively charged depending on their size

d)

positively charged

3.

Stirred-tank bioreactors have been designed for

a)

Purification of the product

b)

Ensuring anaerobic conditions in the culture vessel

c)

availability of oxygen throughout the process

d)

Addition of preservatives to the product

5%

4.

 

which of the following is not a feature of plasmids?

a)

Single stranded

b)

Independent replication

c)

Transferable

d)

Circular structure

5.

The DNA molecule to which the gene of interest is integrated for cloning is called

a)

Transformer

b)

Carrier

c)

Vector

d)

Template

6.

The cutting of DNA at specific locations became possible with the discovery of

a)

Selectable markers

b)

Probes

c)

Restriction enzymes

d)

Ligases

7.

What is the purpose of the polyethylene glycol method?

a)

biodiesel production

b)

energy production from sewage

c)

gene transfer without a vector

d)

seedless fruit production

8.

What is a product of biotechnology?

a)

transgenic crop (GM crop)

b)

humulin

c)

biofertilizer

d)

All of these

9.

What enzyme joins the ends of two strands of nucleic acid?

a)

polymerase

b)

synthetase

c)

ligase

d)

helicase

10.

When separated on an agarose gel, DNA fragments can be visualized after staining with?

a)

Acetocarmine

b)

Aniline blue

c)

Ethidium bromide

d)

Bromophenol blue