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Amino Acids, Peptides, and Proteins

Total questions: 5

Worksheet time: 3mins

Name
Class
Date
1.

Which amino acid has three pKa values?

a)

leucine

b)

proline

c)

glycine

d)

threonine

e)

cysteine

2.

Which statement is correct about peptides?

a)

Peptides have no α-carboxyl groups.

b)

Peptides have their amino acid sequences

     written from the N-terminus.

c)

Peptides do not have isoelectric points.

d)

Peptides are not biologically active.

e)

Peptide bonds are broken through

     condensation reactions.

3.

A new protein resembling myosin was reported. Unlike myosin, it binds calcium. Its isoelectric point and molecular weight are very similar to those of myosin. Which method would BEST separate the new protein from myosin if those two proteins were in the same buffer solution?

a)

ion-exchange chromatography

b)

size-exclusion chromatography

c)

affinity chromatography  

d)

dialysis

e)

fractionation

4.

What measurement increases during purification of an enzyme?

a)

activity

b)

total protein

c)

specific activity

d)

fraction volume

5.

The amino acid sequences of proteins:

a)

can be used to establish evolutionary relationships

between organisms.

b)

are an example of tertiary structure.

c)

are similar within a given organism.

d)

are also referred to as “consensus sequences.”