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Activity 1.1.6 Review

Total questions: 15

Worksheet time: 8mins

Name
Class
Date
1.

The process of ______ is used to create more copies of a specific sequence of DNA.

a)

PCR

b)

Gel Electrophoresis

c)

Micropipetting

d)

Digestion

2.

Restriction enzymes come from ...

a)

viruses

b)

bacteria

c)

plants

d)

animals

3.

The job of restriction enzymes is to ...

a)

copy the DNA.

b)

cut the DNA at specific locations.

c)

remove the DNA.

d)

organize the DNA.

4.

If a restriction enzyme cuts the DNA 3 times, how many recognition sites are there on this DNA?

a)

1

b)

2

c)

3

d)

4

5.

If a restriction enzyme cuts the DNA 3 times, how many RFLPs were made?

a)

1

b)

2

c)

3

d)

4

6.

If a restriction enzyme makes a cut that is straight up and down through the double strand of DNA, this is called creating ______ ends.

a)

sticky

b)

blunt

c)

straight

d)

organized

7.

The process that separates the cut fragments of DNA by their size and compares them is called ____.

a)

PCR

b)

digestion

c)

gel electrophoresis

d)

restriction enzymes

8.

DNA has a _____ charge.

a)

positive

b)

negative

c)

neutral

d)

unknown

9.

The area on the gel where you load the DNA is called the ____.

a)

wells

b)

positive pole

c)

digestion site

d)

loading site

10.

The larger fragments of DNA will ...

a)

remain closer to the wells.

b)

travel towards the + pole of the gel.

c)

not move at all.

d)

travel farther than the smaller fragments.

11.

To pick up DNA with a micropipette, you need to ...

a)

push the round button to the second stop.

b)

push the green eject tip button.

c)

push the round button to the first stop.

d)

All of the others are correct.

12.

True or False: You need to use a new tip with each new sample of DNA.

a)

True

b)

False

13.

According to this gel, who is most likely the child of the dad?

a)

Child 1

b)

Child 2

c)

Both children are his.

d)

Neither child is his.

14.

Aside from crime scenes, when else can gel electrophoresis be used?

a)

Paternity Testing

b)

Animal Breeding

c)

Analyzing Genetically Modified Plants

d)

All of the other options are true.

15.

True or False: The DNA Ladder or DNA Marker is not an important part of the gel results.

a)

True

b)

False