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Molbio Lec: Beginning, Nucleic & genome quiz

Total questions: 50

Worksheet time: 38mins

Name
Class
Date
1.

The sugar component in RNA is______, while ______in DNA.

a)

Deoxyribose, ribose

b)

Ribose, deoxyribose

c)

Ribose, bases

d)

Phosphate, ribose

2.

Nucleoside is a pyrimidine or purine base

a)

Ionically bonded to a sugar

b)

Covalently bonded to a sugar

c)

Both covalently and hydrogen bonded to a sugar

d)

Hydrogen bonded to a sugar

3.

The glycosidic bonds in DNA and RNA

a)

Free to rotate over about 180 degrees

b)

Connect the sugar to the base

c)

Stabilize Watson-crick H-bonds

d)

Can be hydrolyzed by -OH ion

4.

A nucleotide consists of:

a)

Sugar and phosphate

b)

Paired bases

c)

all of these

d)

Sugar, base and a phosphate

5.

Which of the following is found on RNA but not DNA?

a)

Phosphate

b)

Uracil

c)

 

Adenine

d)

Deoxyribose

6.

The monomeric unit of nucleic acid is called_____.

a)

 

Pyrimidines

b)

Purines

c)

 

Nucleosides

d)

 

Nucleotides

7.

Which of the following pyrimidine base is found in RNA but not in DNA?

a)

Guanine

b)

 

Thymine

c)

Adenine

d)

 

Uracil

8.

Name those purine bases which are commonly found in DNA and RNA?

a)

Cytosine and guanine

b)

Adenine and thymine

c)

Cytosine and thymine

d)

Adenine and guanine

9.

The primary structure of DNA and RNA proceeds in which direction?

a)

 

3’ to 5’

b)

 

5’ to 3’

c)

 

4’ to 6’

d)

5' to 5'

10.

Which bond is used to stabilize the double helix of DNA?

a)

Covalent bond

b)

Hydrophobic bond

c)

 

Ionic bond

d)

Hydrogen bond

11.

Which of these statements are incorrect according to chargaff’s rules?

a)

The composition of DNA remains the same in all species

b)

The molar ratio of A to T or C to G = 1

c)

The sum of purine = sum of pyrimidine

d)

 

[A] + [T] = [C]+ [G]

12.

The sugar molecule present in nucleotide is

a)

 

Pentose

b)

 

Tetrose

c)

 

Triose

d)

Hexose

13.

True/False: Purine and pyrimidines are different molecules.

a)

True

b)

false

14.

Which best explains the function of the sequence of nucleotides?

a)

 

All of the choices

b)

It carries the genetic information

c)

 

It provides energy

d)

It determines the shape of the DNA molecule.

15.

The following statements are true, except:

a)

Hydrogen bonds are weak bonds

b)

 

All of the choices are correct.

c)

Hydrogen bonding between bases is referred to as complementary base pairing

d)

 

Adenine normally pairs with thymine by three H-bonds while guanine and cytosine by two H-bond

16.

True about base stacking, except:

a)

Paired flat bases tend to stack on top of one another by means of a helical twist.

b)

All of the choices.

c)

This eliminates any gaps between the phosphate and sugar.

d)

Excludes the amount of water from the interior of the double helix.

17.

The _________ carries a message in a form that can be read by DNA-binding protein while _________ is less informative.

a)

 

Major groove; phosphate + ribose

b)

Minor groove; two ribose + nitrogenous bases

c)

 

Major groove; Minor groove

d)

Minor groove; Major groove

18.

True about B-DNA:

I. Right-handed helix

II. 5 bases per turn

III. Occurs under high humidity and low salt

IV. Occurs under Low humidity and high salt

V. Form a zigzag structure

a)

I, II, IV

b)

I, II, III, V

c)

I, II, III

d)

I, II, II, IV

19.

True/False: The stress present within supercoil DNA molecules leads to localized denaturation.

a)

 

True

b)

 

False

20.

True about H1 histone

a)

 

Both answers are correct

b)

 

Tightly bound to DNA

c)

 

Easy to remove from chromatin

21.

This principal type of RNA transports amino acids to the site of protein synthesis.

a)

 

tRNA

b)

miRNA

c)

 

mRNA

d)

rRNA

22.

True about Gregor Mendel, except:

a)

Bred different varieties of Pisum sativum.

b)

 

Law of combination of different character.

c)

All of the choices are correct

23.

The organism was used by George Beadle and Edward Tatum in their experiment. 

a)

 

Neurospora crassa

b)

Saccharomyces spp

c)

Both Neurospora and Saccharomyces

d)

Streptococcus pneumoniae

24.

True about the Hershey-chase experiment:

a)

They incubated the Staphylococcus aureus with labeled phage.

b)

They designed the experiment to determine whether the protein or DNA carries genetic information to make a new bacteriophage.

c)

Selective labeled phage DNA with 32-phosphorus and phage DNA with 35-sulfur.

d)

 

All of the choices are correct.

25.

The study of the molecular structure of DNA and the information it encodes.

a)

Molecular biology

b)

Molecular medicine

c)

Molecular phylogenetics

d)

Molecular diagnostics

26.

He demonstrates a heritable transforming principle that transmits the ability of bacteria to cause pneumonia in mice.

a)

 

Frederick Griffith

b)

William Bateson

c)

Phoebus Aaron Levene

d)

George Beadle

27.

He proposes his germ plasm theory and challenges the widely held idea that acquired characteristics can be inherited.

a)

Hugo DeVries

b)

 

Walter Flemming

c)

Gregor Mendel

d)

August Weismann

28.

He shows that the amount of bases Adenine and Thymine and Guanine and Cytosine are equal.

a)

Rosalind Franklin

b)

Alfred Hershey

c)

Maurice Wilkins

d)

Erwin Chargaff

29.

They demonstrate that Griffith’s principle is not a protein but DNA and suggest that it may function as the genetic material.

I. Colin MacLeod

II. Oswald Avery

III. Martha Chase

IV. Maurice Wilkins

V. Maclyn McCarty

a)

 

I, II, IV

b)

I, III, IV

c)

I, II, V

d)

II, III, IV

30.

He characterizes and names the compounds ribonucleic acid and deoxyribonucleic acid and a trinucleotide structure of DNA.

a)

Phoebus Aaron Levene

b)

 

James D. Watsons and Francis Crick

c)

Rudolf Signer

d)

 

Rosalind Franklin

31.

Genomic organization refers to the linear order of DNA elements and their division into chromosomes.

a)

true

b)

false

32.

The distinct zig-zag appearance of the chromatin fibre is due to ____________

a)

Histone core

b)

Histone H1

c)

Linker DNA

d)

 

Nucleosome

33.

Which of the following regions promote histone – DNA association?

a)

 

A, G

b)

 

A, T

c)

G, C

d)

A, C

34.

Genomic DNA exists as single linear pieces of DNA that are associated with a protein called?

a)

nucleoprotein complex

b)

helical complex

c)

nucleoprotein chromosomes

d)

helical DNA

35.

What marks the difference between the solenoid and the zig-zag models of 30 nm fibres?

a)

Linker histone molecule

b)

10 nm fibre

c)

 

Linker DNA

d)

Nucleosome structure

36.

True/ False: Genome organization does not refer to the 3D structure of chromosomes and the positioning of DNA sequences within the nucleus.

a)

 

True

b)

False

37.

True/ False: A very small fraction of the genome is also found in a 16,000 base pair circular piece of DNA that is found in the mitochondria.

a)

True

b)

False

38.

How many types of histone molecules are found in nature?

a)

5

b)

4

c)

2

d)

3

39.

Only 1.2% of the mammalian genome thus encodes for protein function

a)

True

b)

False

40.

In the beads on a string model, the bead is made up of __________

a)

6 histone proteins and DNA

b)

6 histone proteins

c)

 

8 histone proteins and DNA

d)

8 histone proteins

41.

Nucleosome is made up of _______________________

a)

RNA, histone core protein

b)

DNA, histone core protein, linker H1

c)

DNA, histone core protein

d)

RNA, histone core protein, linker H1

42.

With respect to Histone H1 DNA packaging which of the following is false?

a)

H1 binds to two DNA helices at once

b)

H1 stabilizes higher order chromatin structures

c)

H1 induce 10 nm fibre formation

d)

H1 binding gives nucleosome a defined angle

43.

With respect to the solenoid model of nucleosome, which of the following is true?

a)

 

Structure supported by X – ray diffraction

b)

Helical pitch of approximately 13 nm

c)

Helical model

 

d)

 

8 nucleosomes per turn

44.

What marks the difference between the solenoid and the zig-zag models of 30 nm fibres?

a)

 

Linker DNA

b)

Nucleosome structure

c)

10 nm fibre

d)

Linker histone molecule

45.

The positioning of nucleosome is beneficial.

a)

True

b)

False

46.

The unpacked stretches of DNA are the extra chromosomal load found in the eukaryotic genome.

a)

True

b)

False

47.

Histones have a high content of negatively charged amino acids

a)

True

b)

False

48.

eukaryotic genome has genes in the form of____________

a)

 

chromosome

b)

 

all of these

c)

nucleosome

d)

 

chromatin

49.

Approximately how many genes does the human genome contain?

a)

10,000-15,000

b)

50,000-60,000

c)

70,000-80,000

d)

20,000-25,000

50.

What is the approximate size of the short region of the DNA double helix?

a)

 

4nm

b)

 

2nm

c)

3nm

d)

0.2nm

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