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DNA Replication

Total questions: 15

Worksheet time: 8mins

Name
Class
Date
1.

Which of the following correctly pairs the DNA replication enzyme with its function

a)

Topoisomerases work ahead of the replication fork to prevent supercoiling.

b)

DNA polymerase I opens up the DNA at the replication fork.

c)

Helicase seals gaps between DNA fragments.

d)

DNA primase extends primers by adding nucleotides to the 3'3′3, prime end.

2.

The method of DNA replication is known as:

a)

Dispersive

b)

Semi-conservative

c)

Conservative

d)

Complementary

3.

The enzyme used to separate the double helix into 2 single strands is:

a)

Nuclease

b)

Primase

c)

Helicase

d)

DNA polymerase III

4.

Bacterial chromosome....

a)

has linear DNA

b)

located inside nucleus

c)

associates with large amount of proteins

d)

has circular DNA

5.

In a semi-conservative model of DNA replication, which of the following is true?

a)

The daughter DNA molecule consists of 2 parent strands

b)

The daughter DNA molecule consists of an uneven mix of old and new strands

c)

The daughter DNA molecule consists of 1 parent strand and 1 new strand

d)

The daughter DNA molecule consists of 2 newly formed strands

6.

In a molecule of double-stranded DNA, the amount of Adenine present is always equal to the amount of

a)

Cytosine

b)

Guanine

c)

Thymine

d)

Uracil

7.

What prevents DNA strands from reannealing at the replication fork?

a)

Helicase

b)

Topoisomerase

c)

Ligase

d)

Single-stranded binding proteins

8.

Which enzyme joins together Okazaki fragments?

a)

DNA pol I

b)

Helicase

c)

Ligase

d)

Primase

9.

The DNA sequence of the parent strand is 5'-ATGCTCAGCT-3'. What is the sequence of the daughter strand?

a)

3'-UACGAGUCGA-5'

b)

5'-UACGAGUCGA-3'

c)

3'-TACGAGTCGA-5'

d)

5'-TACGAGTCGA-3'

10.

In what phase of the cell cycle does DNA replication take place?

a)

G1

b)

G2

c)

S

d)

M

11.

Which template strand(s) will be used to synthesize new DNA strand(s) in short fragments

a)

A

b)

B and C

c)

A and D

d)

D

12.

Which of the following is NOT true about DNA polymerase III?

a)

Replaces RNA primer at the lagging strand with DNA nucleotide

b)

Requires short primer before adding the new DNA nucleotide

c)

Adds DNA nucleotide at a free 3' end of an existing chain

d)

Cannot initiate synthesis of a new DNA strand

13.

Why would it be important to replicate DNA before a cell divides in mitosis or meiosis?

a)

In order for the DNA to be contained in the nucleus.

b)

In order for the cell to re-order the DNA sequencing in the new cells.

c)

In order for the cell to be able to increase in size.

d)

In order for genetic information to be transferred into daughter cells.

14.

Which of the following is correct with respect to DNA replication?

a)

Enzyme Helicase is used to stabilize the ssDNA

b)

Enzyme DNA Pol. I is the main polymerizing enzyme

c)

Enzyme ligase is used to employ RNA primers

d)

Okazaki fragments are made up of deoxynucleotides

15.

The last stage of chromatin packing produces...

a)

nucleosome

b)

histones

c)

looped domain

d)

metaphase chromosome