WorksheetsMBT ELISA & Antibodies
Total questions: 20
Worksheet time: 11mins
What term describes a molecule on a pathogen to which an antibody can bind?
(a)
Antibody structure is commonly
Z shaped
Y shaped
W shaped
T shaped
When a positive + result occurs in an ELISA test, then one could conclude that...
a + result always means illness
the antibody is present
it is a false + and no illness
an enzyme is present
During an ELISA lab, you prepare a set of dilutions...why?
What is a primary antibody?
Patient's antibody and initial step in ELISA
Antibody with bound enzyme
The most important antibody
a chicken antibody
The immune system of human may respond to the epitopes on the surface of an invading organism by
Inhibiting the action of B cells
Disrupting the mitochondria of the invading organism
Producing antibodies
Altering the DNA sequence of the invading organisms
Antibodies consist of
A light chain and 2 heavy chains
2 light chains and 2 heavy chains
2 light chains and a heavy chain
All options are true because there are many types of antibodies
The part of an antigen molecule to which an antibody attaches itself is called a(n)
(a)
What kind of antibody isotope can pass from mother to fetus?
IgM
IgG
IgA
IgD
In an ELISA test, what does the secondary antibody recognize?
The antigen
The antibody
The antigen-antibody complex
Why is a colorless substrate added after the final washing step of an ELISA lab?
To remove any unbound antibodies
To cause a color change
To wash the well
To indicate the presence of the primary antibody
Which of the following cannot be determined by an ELISA test?
Concentration of antigens
Presence of pathogen
Genetic sequence of a pathogen
Presence of protein
What are Monoclonal antibodies ?
An antibody produced by the body.
An antibody produced by a single clone of cells or cell line and consisting of identical antibody molecules.
An antibody produced by a single clone of cells or cell line and consisting of many different antibody molecules.
An antibody produced in a lab
Monoclonal antibodies can be used for...
Cancer treatment
Pregnancy detection
Tissue transplants
Autoimmune disorders
5.) In what order should you add the reagents to perform an indirect ELISA test?
a.) Primary antibody, antigen sample, enzyme-linked secondary antibody, substrate
b.) Antigen sample, primary antibody, enzyme-linked secondary antibody, substrate
c.) Substrate, enzyme-linked secondary antibody, antigen samples
d.) Primary antibody, enzyme-linked secondary antibody, antigen samples, substrate
Put the steps of an ELISA assay in order.
make serial dilutions of antigen of interest for comparison
add samples to be tested
add primary Ab to all wells, let develop then wash
add secondary Ab with attached enzyme to all wells, develop and then wash
add substrate to all wells
