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Worksheets3 Isolation & Cultivation of Mcgs - MicroCore Review Center
Total questions: 10
Worksheet time: 5mins
Which of the following does not describe a pure culture?
Contains a single species of microorganism
Axenic culture
Contains a microorganism with cells having the same Gram-reaction
All of them describe a pure culture
Which of the following is described as the increase in microbial population by providing their nutritional and physical requirements?
Cultivation
Isolation
Preservation
Serial dilution
Which culture media (according to its physical state) is the best to use for studying motility?
Liquid
Solid
Semi-solid
Solid, Liquid, and Semi-solid
Which culture media has yeast extract?
Complex
Synthetic
Chemically-defined media
Both complex and synthetic
Which of the following types of culture media allows the growth of a specific type/group of microorganisms only, while inhibiting the growth of others?
General Purpose
Selective
Differential
All of the aforementioned
Which of the following statements is incorrect?
EMBA is both selective and differential.
TCBSA is both selective and differential
TCBSA cannot differentiate between species of Vibrio.
EMBA can differentiate between strong and weak lactose fermenters.
Eosin Methylene Blue Agar (EMBA) contains digested meat proteins as a source of organic nutrients. Two indicator dyes, eosin and methylene blue, inhibit the growth of gram-positive bacteria and distinguish between lactose fermenting and non-lactose fermenting organisms. Lactose fermenters form metallic green or deep purple colonies, whereas non-lactose fermenters form completely colorless colonies. EMB agar is an example of which of the following?
Selective media
Differential Media
Selective and Differential
Selective, Differential, and Complex
Selective, Differential, and Defined
Which isolation technique is best to use for samples with a low population?
Membrane filtration
Serial dilution
Isolation is impossible
Both membrane filtration and serial dilution
Which of the following preservation methods is best used for the long-term storage of microbial cultures?
Refrigeration
Subculturing
Mineral Oil Overlay
Lyophilization
Which culture preservation method is based on the principle of limiting the availability of oxygen, thereby decreasing the metabolic rate of the organism?
Subculturing
Mineral Oil Overlay
Freeze-drying
Freezing with liquid nitrogen
