wayground logo

Free Printable Worksheets

NEW

Font size

S
M
L
XL
Worksheets

3 Isolation & Cultivation of Mcgs - MicroCore Review Center

Total questions: 10

Worksheet time: 5mins

Name
Class
Date
1.

Which of the following does not describe a pure culture?

a)

Contains a single species of microorganism

b)

Axenic culture

c)

Contains a microorganism with cells having the same Gram-reaction

d)

All of them describe a pure culture

2.

Which of the following is described as the increase in microbial population by providing their nutritional and physical requirements?

a)

Cultivation

b)

Isolation

c)

Preservation

d)

Serial dilution

3.

Which culture media (according to its physical state) is the best to use for studying motility?

a)

Liquid

b)

Solid

c)

Semi-solid

d)

Solid, Liquid, and Semi-solid

4.

Which culture media has yeast extract?

a)

Complex

b)

Synthetic

c)

Chemically-defined media

d)

Both complex and synthetic

5.

Which of the following types of culture media allows the growth of a specific type/group of microorganisms only, while inhibiting the growth of others?

a)

General Purpose

b)

Selective

c)

Differential

d)

All of the aforementioned

6.

Which of the following statements is incorrect?

a)

EMBA is both selective and differential.

b)

TCBSA is both selective and differential

c)

TCBSA cannot differentiate between species of Vibrio.

d)

EMBA can differentiate between strong and weak lactose fermenters.

7.

Eosin Methylene Blue Agar (EMBA) contains digested meat proteins as a source of organic nutrients. Two indicator dyes, eosin and methylene blue, inhibit the growth of gram-positive bacteria and distinguish between lactose fermenting and non-lactose fermenting organisms. Lactose fermenters form metallic green or deep purple colonies, whereas non-lactose fermenters form completely colorless colonies. EMB agar is an example of which of the following?

a)

Selective media

b)

Differential Media

c)

Selective and Differential

d)

Selective, Differential, and Complex

e)

Selective, Differential, and Defined

8.

Which isolation technique is best to use for samples with a low population?

a)

Membrane filtration

b)

Serial dilution

c)

Isolation is impossible

d)

Both membrane filtration and serial dilution

9.

Which of the following preservation methods is best used for the long-term storage of microbial cultures?

a)

Refrigeration

b)

Subculturing

c)

Mineral Oil Overlay

d)

Lyophilization

10.

Which culture preservation method is based on the principle of limiting the availability of oxygen, thereby decreasing the metabolic rate of the organism?

a)

Subculturing

b)

Mineral Oil Overlay

c)

Freeze-drying

d)

Freezing with liquid nitrogen