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REINFORCEMENT 1 DB014 - Mutation & Recombinant DNA Technology

Total questions: 8

Worksheet time: 4mins

Name
Class
Date
1.

In sickle cell anaemia, glutamic acid is replaced with valine.

Identify the type of gene mutation that has occurred.

a)

Base deletion.

b)

Base insertion.

c)

Inversion.

d)

  Base substitution.

2.

A _____________ is a nucleotide base substitution that results in new amino acid. 

a)

nonsense mutation.         

          

b)

  silent mutation. 

c)

missense mutation.        

d)

point mutation. 

3.

Cri du chat is a chromosomal condition that results when a piece of number 5 chromosome is missing.  

What type of chromosomal mutation that has occurred?

a)

Deletion

                  

            

b)

Inversion

c)

Duplication

d)

Translocation

4.

What type of gene mutation has occurred when UAU is changed to UCC?  

a)

Base substitution.  

         

 

b)

Base insertion.

c)

Inversion. 

d)

Base deletion.  

5.

EcoR1 is an example of enzyme that recognizes and cuts DNA molecules at a specific recognition site. This is an example of a  

a)

DNA ligase

b)

DNA polymerase

c)

RNA polymerase

d)

  restriction enzyme

         

6.

The mechanism where a recombinant DNA is introduced into host cells is called 

a)

transduction. 

b)

  conjugation.

c)

amplification. 

d)

transformation.

7.

Which pair of enzymes are needed in gene technology?      

a)

RNA polymerase and DNA ligase

b)

DNA polymerase and DNA ligase

c)

DNA ligase and restriction enzyme

d)

RNA polymerase and restriction enzyme

8.

A small and circular DNA that is used as a cloning vector is  

a)

Escherichia coli

         

b)

  plasmid

c)

target DNA

d)

bacteria