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Gene Expression from Gene to Protein

Total questions: 10

Worksheet time: 5mins

Name
Class
Date
1.

Which of the following statements correctly defines the process of gene expression?

a)

Gene expression is the process by which proteins direct the synthesis of DNA.

b)

Gene expression is the process by which DNA directs the synthesis of proteinsGene expression is the process by which DNA directs the synthesis of proteins

c)

Gene expression is the process by which proteins direct the synthesis of RNA.

d)

Gene expression is the process by which RNA direct the synthesis of DNA.

2.

The genetic code is essentially the same for all organisms. Based on this information, one can logically assume which of the following statements to be correct?

a)

A gene from an organism can theoretically be expressed by any other organism

b)

Gene expression differs considerably in different organisms

c)

DNA was the first genetic material

d)

The same codons in different organisms translate into the different amino acids.

3.

Which of the following processes only occurs in eukaryotic gene expression?

a)

mRNA, tRNA, and rRNA are transcribed

b)

RNA polymerase binds to the promoter.

c)

A poly-A tail is added to the 3' end of an mRNA, and a cap is added to the 5' end.

d)

Transcription can begin as soon as translation has assembled the first few amino acids in the polypeptide

4.

Transcription in eukaryotes requires which of the following in addition to RNA polymerase?

a)

the protein product of the promoter

b)

start and stop codons

c)

ribosomes and tRNA

d)

several transcription factors

5.

In what way could a point mutation in DNA make a difference in the function of a protein?

a)

It might result in a chromosomal translocation

b)

It might exchange one stop codon for another stop codon

c)

It might exchange one serine codon for a different serine codon.

d)

It might substitute a different amino acid in the protein, which could change its function.

6.

A particular triplet of bases in the template strand of DNA is 5'-AGT-3'. The corresponding codon for the mRNA transcribed is

a)

3'-UCA-5'

b)

3'-UGA-5'

c)

5'-TCA-3'

d)

3'-ACU-5'

7.

Which of the following statements best describes the function of the TATA box in eukaryotic promoters?

a)

It is the recognition site for a specific transcription factor

b)

It sets the reading frame of the mRNA

c)

t is the recognition site for ribosomal binding

d)

It signals the end of the nucleotide sequence of the gene

8.

Which of the following is a function of a poly-A tail in mRNA?

a)

It adds the modified guanine to the 3' end of the mRNA.

b)

It indicates the site of translational termination

c)

It is a sequence that codes for the binding of RNA polymerase to the DNA

d)

It helps protect the mRNA from degradation by hydrolytic enzymes

9.

What is the function of the release factor in translation?

a)

It separates tRNA in the A site from the growing polypeptide

b)

It binds to the stop codon in the A site in place of a tRNA

c)

It binds to the stop codon in the A site in place of a tRNA

d)

It releases the ribosome from the ER to allow polypeptides into the cytosol

10.

Which small-scale mutation would be most likely to have the least negative effect on the function of a protein?

a)

a base substitution

b)

a base deletion near the start of a gene

c)

a base deletion near the end of the coding sequence, but not in the terminator codon

d)

deletion of three bases near the start of the coding sequence, but not in the initiator codon