WorksheetsTA Section 2 (2/14)
Total questions: 22
Worksheet time: 14mins
All of the following are true EXCEPT:
Gene expression involves transcription and translation
Transcription copies DNA to RNA
Translation copies RNA to protein
All RNAs are translated into protein
These processes occur in the nucleus:
Polyadenylation of 3'UTR
5' RNA capping
Protein Translation
RNA splicing
RNA degradation
Ribose and deoxyribose differ at which carbon?
1'
2'
3'
4'
RNA capping, splicing, and polyadenylation factors can only bind to the tail of RNA Polymerase II when the tail is ____ by TFIIH.
phosphorylated
acetylated
methylated
cleaved
The RNA transcript matches the sequence of the
non-coding strand
coding strand
both strands
neither strand
In the pre-mRNA, sequences that encode protein are (a) and the non-coding sequences are (b) .
The ribosome is made up of protein and ribozymes, which are...
RNAs that carry an anticodon sequence and an amino acid
proteins that specifically bind RNA
RNAs with catalytic functions
The energy for phosphodiester bond formation comes from
peptide bonds
the phosphoanhydride bond of the nucleotide
RNA polymerase
no energy is required
The spliceosome catalyzes the removal of introns from the pre-mRNA. It is made up of snRNPs. snRNPs are...
short RNA sequences
small proteins
nucleases
RNA-protein complexes
RNA polymerase binds at the
Transcription start site
Promoter
Terminator sequence
Activator
During RNA splicing, the 2'OH of the ______ attacks the 5' splice site, forming a lariat structure (containing the intron) that will be released.
branch point guanidine
branch point adenine
branch point cytosine
branch point thymine
What functional group is added to RNA polymerase II to begin transcription in eukaryotes
acetyl
methyl
phosphate
hydroxyl
Each of the 64 possible codons (3 nucleotides each) encode an amino acid to be added to the growing protein chain.
True
False
cDNA libraries...
are complementary DNA libraries
contain introns
are generated using reverse transcriptase
contain all genes
RNAs can only be translated into protein by one ribosome at a time.
True
False
The third cycle of PCR produces how many double-stranded DNA molecules?
4
6
8
10
Different cell types
Only contain certain genes
Contain all genes
Express all genes
Have the same proteins
Alternative RNA splicing allows a single gene to code for multiple forms of a protein by changing the ____ that are translated.
order of exons
order of introns
number of exons
number of introns
Which of the following transcription regulators is NOT used to produce pluripotent stem cells?
oct4
Sox2
mer7
klf4
miRNAs are bound to proteins called the (a) complex, and (b) RNAs by inducing their (c) .
During translation: the ___ subunit of the ribosome binds the mRNA and matches tRNAs to the codons, and the ___ subunit catalyzes formation of peptide bonds on the growing protein chain.
large, small
small, large
RNA, protein
protein, RNA
Ubiquitin tags proteins for ____ by the proteosome
degradation
methylation
translation
RNA binding
