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Enzyme Kinetics Practice

Total questions: 24

Worksheet time: 9mins

Name
Class
Date
1.

A protein is made up of:

a)

long chain of amino acids

b)

bilayer of lipids

c)

long chain of nucleotides

d)

adenine, guanine, cytosine and uracil

2.

This is a substance that can be added to increase the reaction:

a)

Catalyst

b)

Buffer

c)

Cofactor

d)

Temperature

3.

What does NAD stands for:

a)

Nomenclature association disease

b)

Nucleotide amine deoxyde

c)

Nicotinamide adenine dinucleotide

d)

Adenosin triphosphate

4.

Cu 2+ and enzyme is called:

a)

Prostetic group

b)

Metallic enzyme

c)

Co-enzyme

d)

Essential metal

5.

Specific region on the enzyme's surface where the substrate molecule binds and undergoes a chemical reaction:

a)

Active site

b)

Allosteric site

c)

Binding site

d)

Regulatory site

6.

What EC number for ligases?

a)

1

b)

6

c)

3

d)

4

7.

In which type of inhibition does the inhibitor binds to the ES complex:

a)

uncompetitive

b)

competitive

c)

non competitive

d)

all of them

8.

This is the name for the series of reactions that occur within cells of living organisms to sustain life

(a)  

9.

Competitive inhibitors bind to:

a)

Substrate

b)

Free enzyme

c)

ES complex

d)

Allosteric site

10.

This graph represents a:

a)

Lineweaver-Burk

b)

Michaelis-Menten

c)

Dixon

d)

Arrhenius

11.

This plot represents a ______ inhibitor reaction

a)

Competitive

b)

Uncompetitive

c)

Non-competitve

d)

Mixed

12.

Name of molecules formed in glycolisis

a)

ATP and pyruvate

b)

ATP and FADH2

c)

pyruvate and glyceraldehyde

d)

ATP and H2O2

13.

In Krebs cycle, first pyruvate has to be transformed to:

a)

acetyl coA

b)

citrate

c)

lactic acid

d)

oxaloacetate

14.

Electron transport chain occurs in:

a)

cytoplasm

b)

mitochondria

c)

ribosome

d)

nucleus

15.
a)

symport

b)

antiport

c)

uniport

16.

What is the name of the membrane transport that shows the image?

a)

simple diffusion

b)

Passive transport

c)

Active transport

d)

Endocytosis

17.

This is factor does not affect enzyme rate:

a)

pH

b)

temperature

c)

Substrate

d)

Buffer

18.

Enzymatically speaking, excessively high temperatures can lead to:

a)

denaturation

b)

activation

c)

inhibition

d)

polymerization

19.

This equation describes the temperature dependence of the rate of a chemical reaction:

a)

Michaelis-Menten

b)

Kcat

c)

Ki

d)

Arrhenius

20.

Most activators are:

a)

cations

b)

complex molecules

c)

enzymes

d)

substrates

21.

What is the change in the affinity for binding of a ligand or substrate that is caused by the binding of another ligand away from the active site?

a)

allosterism

b)

catabolism

c)

metabolism

d)

competitive inhibition

22.

This is a way to release proteins inside the cell:

a)

sonication

b)

osmosis

c)

endocytosis

d)

centrifugation

23.

What is the name of the purification method were the proteins are prevented to form hydrogen bonds with water and the salt facilitates their interaction with each other forming aggregates that afterward precipitate out of solution

a)

ammonium sulfate precipitation

b)

dialysis

c)

differential precipitation

d)

ultracentrifugation

24.

What it the name of this method were in mixture applied onto the surface or into the solid, and fluid stationary phase is separating from each other while moving with the aid of a mobile phase:

a)

Chromatography

b)

Dialysis

c)

Ammonium sulfate precipitation

d)

SDS page