WorksheetsMol Bio Quiz4
Total questions: 6
Worksheet time: 5mins
What is the correct sequence of steps involved in nucleic acid isolation?
Nucleic acid precipitation -> Removal of cell debris -> Lysis -> Dissolving nucleic acid -> Protein digestion
Lysis-> Removal of cell debris -> Nucleic acid precipitation -> Protein digestion -> Dissolving nucleic acid
Lysis -> Protein digestion -> Removal of cell debris -> Nucleic acid precipitation -> Dissolving nucleic acid
Lysis -> Removal of cell debris -> Protein digestion -> Dissolving DNA -> Nucleic acid precipitation
In a real-time PCR reaction, linear (TaqMan) probes generate signal when cleaved due the 5' to 3' exonuclease activity of DNA extension polymerase and molecular beacons generate signal when they bind to their complementary sequence. In which step of the PCR cycling will you keep the camera on to detect nucleic acid amplification?
TaqMan probes: Initial extension
Molecular beacons: Final extension
TaqMan probes: Denaturation
Molecular beacons: Annealing
TaqMan probes: Extension
Molecular beacons: Annealing
TaqMan probes: Extension
Molecular beacons: Extension
You are to design a PCR assay to test for COVID. List all the enzymes you will need for the assay.
Which of the following techniques can be used to visualize genomic DNA which is 200kb long, using lab equipment you have used in this semester?
Using DNA probes and measuring fluoresence
Gel electrophoresis
SDS PAGE
Pulsed field gel electrophoresis
The sequence of a target gene you are testing for is given below:
5'GGTAGCTTAGCTGACTGCCATGGCATG........GGTCGATCGTTAACTGGATCCGGATGCAT3'
The sequence mapped for primer design is highlighted in red. Write the correct sequence for the primer set in 5' to 3' direction.
AATCGACTGACGGT
AND
AATTGACCTAGGCCTA
TGGCAGTCAGCTAA
AND
AATTGACCTAGGCCTA
TTAGCTGACTGCCA
AND
ATCCGGATCCAGTTAA
ACCGTCAGTCGATT
AND
ATCCGGATCCAGTTAA
60-65 Deg C
LAMP
Thermal cycling
PCR
60-64 Deg C
HDA
35-37 Deg C
RPA
