Font size
WorksheetsSerological and Molecular Detection of Bacerial Infections
Total questions: 32
Worksheet time: 32mins
- host and microbes live together long term
- indigenous microbiota
symbiotic
commensalistic
mutualistic
parasitic
- no benefit or harm to either organism
symbiotic
commensalistic
mutualistic
parasitic
- both host and microbes benefit
symbiotic
commensalistic
mutualistic
parasitic
- microbes cause harm to the host
symbiotic
commensalistic
mutualistic
parasitic
organism's ability to establish an infection
infectivity
pathogenicity
virulence
ability of an organism to cause disease
infectivity
pathogenicity
virulence
extent of pathology caused by an organism when it infects a host
infectivity
pathogenicity
virulence
- Growth on broth or solid media
- Major means of diagnosis, but may take time or may not be possible
culture of the causative agent
microscopic examintaion
detection of bacterial antigens
molecular detection of bacterial DNA or RNA
serology
- gram stain or special stain
culture of the causative agent
microscopic examintaion
detection of bacterial antigens
molecular detection of bacterial DNA or RNA
serology
- rapid testing by ELISA, LFA, or LA
culture of the causative agent
microscopic examintaion
detection of bacterial antigens
molecular detection of bacterial DNA or RNA
serology
- Can obtain results in a few hours with PCR
culture of the causative agent
microscopic examintaion
detection of bacterial antigens
molecular detection of bacterial DNA or RNA
serology
- detects antibodies to bacterial antigens
culture of the causative agent
microscopic examintaion
detection of bacterial antigens
molecular detection of bacterial DNA or RNA
serology
- most common
- can include fever, chills, headache
Pharyngitis
Pyoderma
Toxic shock syndrome
Necrotizing fascilitis
Treated with antibiodics
- include by streptococcus pyogenes exotoxin that can act as superantigens that produces overproduction of cytokine
- usually related to tampons
Pharyngitis
Pyoderma
Toxic shock syndrome
Necrotizing fascilitis
Treated with antibiodics
- can occur when it is skin infection invades the muscles in he extremities or in thr throat
- caused by streptococcal X toxins that can induce a rapidly spreading infection that can result to ischemia
Pharyngitis
Pyoderma
Toxic shock syndrome
Necrotizing fascilitis
Treated with antibiodics
- develops 1 to 3 weeks after pharyngitis or tonsillitis in 2% to 3% of infected individuals
- most likely caused by immune responses to streptococcal antigen that cross - react with human heart tissue
Acute rheumatic fever
Post streptococcal glomerulonephritis
- deposits of immune complexes containing streptococcal antigens in glomeruli
Acute rheumatic fever
Post streptococcal glomerulonephritis
- Nephelometric method currently used that measure lights scatter produced by immune commplexes containing streptolysin antigen
- titer elevated in 85% of patients with acute rheumatic fever
Antistrptolysin O (ASO)
Anti-DNase B
Streptozyme test
- produced by both rheumatic fever and impetigo patients
- Tested by EIA and nephelometric methods
- Test the ability of DNA's antibodies in the patient serum
Antistrptolysin O (ASO)
Anti-DNase B
Streptozyme test
- patient will ingest a radioactive carbon (14C) or nonradioactive 13C
- important in determining whether the bacteria have been eradicated by antimicrobial therapies
Detect urease in stimach biopsy (CLO test)
Urea breath test
H pylori antigens
H pylori antibodies
- yellow gel with turn into hot pink as a result of increase in AH
- majority of test turn positive within 20 minutes
Detect urease in stimach biopsy (CLO test)
Urea breath test
H pylori antigens
H pylori antibodies
- determine whether the bacteria have been eliminated after treatment
Detect urease in stimach biopsy (CLO test)
Urea breath test
H pylori antigens
H pylori antibodies
active or recurrent infection
O antigen
H antigen
A or B antigen
O and A or B antigen
- past infection
- recent immunizations
- exposure
- Covalescent
O antigen
H antigen
A or B antigen
O and A or B antigen
- Paratyphoid fever
O antigen
H antigen
A or B antigen
O and A or B antigen
- mixed infection
O antigen
H antigen
A or B antigen
O and A or B antigen
- gram positive bacteria
- commonly cause acute infections of the upper respiratory tract and skin
Group A streptococci
Helicobacter pylori
Mycoplasma pneumoniae
Rickettsia
- gram negative
- urease producing bacerium that cause gastric and diodenal ulcers
- can lead to gastric carcinoma or MALT tumors
Group A streptococci
Helicobacter pylori
Mycoplasma pneumoniae
Rickettsia
- tiny bacteria that lack cell wall
- detection: PCR and serologic assays
Group A streptococci
Helicobacter pylori
Mycoplasma pneumoniae
Rickettsia
- obligate intracellular gram - negatice bacteria
- transmitted by arthropods
Group A streptococci
Helicobacter pylori
Mycoplasma pneumoniae
Rickettsia
gold standard for laboratory diagnosis of RMSF
(a)
has its limitation, there can be delay in stat of infection and antibody production
(a)
