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WorksheetsMI Unit 1 Review
Total questions: 21
Worksheet time: 11mins
1.) Which of the following is most responsible for an outbreak?
Antibodies
Pathogens
Genomes
Plasmids
2.) PCR is used to
cut DNA
clone cells
copy DNA sequences
create recombinant DNA
3.) Primers in PCR reactions are made up of
a.) double stranded DNA
b.) single stranded DNA
c.) protein chains
d.) tagged nucleotides
4.) During the ELISA test, the secondary antibody was attached to an enzyme. What happens when that enzyme becomes attached to the substrate?
a.) The enzyme and the substrate reacted with the primary antibody and caused a color change.
b.) The enzyme and the substrate work worked together to destroy the antigen, resulting in a color change.
c.) The enzyme caused the substrate to change, resulting in a color change.
d.) The substrate caused the enzyme to change, resulting in a color change.
5.) In what order should you add the reagents to perform an ELISA test?
a.) Primary antibody, antigen sample, enzyme-linked secondary antibody, substrate
b.) Antigen sample, primary antibody, enzyme-linked secondary antibody, substrate
c.) Substrate, enzyme-linked secondary antibody, antigen samples
d.) Primary antibody, enzyme-linked secondary antibody, antigen samples, substrate
6.) ________ have surface _________ where _________ attach.
a.) antibodies, pathogens, antigens
b.) antigens, antibodies, pathogens
c.) pathogens, antibodies, antigens
d.) pathogens, antigens, antibodies
7.) An outbreak of a disease has been spreading through a small community. Using samples taken from infected patients, which of the following tests BEST enable doctors at the CDC to identify an unknown pathogen from the outbreak?
a.) Determine the sequence of the pathogen's DNA with an automated sequencer and then amplify key genes using PCR.
b.) Determine the sequence of the pathogen's DNA using recombinant DNA methods and then amplify the sequence using PCR.
c.) Use an automated sequencer to determine the nucleotide sequence of the samples, then compare these sequences with known organisms using BLAST.
d.) Use PCR to amplify the genome of the infected patients, then BLAST the genome results.
8.) Which of the following is a medical intervention?
a.) PCR
b.) BLAST
c.) DNA sequencing
d.) All of the above
9.) What career combines molecular biology and computer analysis?
a.) diagnostic imaging
b.) genetics
c.) bioinformatics
d.) microbiology
10.) Place the following processes for using DNA for bacterial identification in order:
1. obtain bacterial DNA by bursting open bacterial cells with enzymes
2. Determine the sequence of bacterial DNA by reading the order of fluorescent nucleotides
3. Use BLAST to match the bacterial sequence to known organisms
4. Use specific primers and PCR to amplify specific pieces of bacterial DNA.
5. Use fluorescent tagged nucleotides in sequencing PCR.
a.) 1, 4, 5, 2, 3
b.) 1, 4, 3, 5, 2
c.) 1, 2, 5, 3, 4
d.) 1, 2, 3, 4, 5
11.) Which structure contains accessory genes such as antibiotic resistance and can be used for recombinant DNA?
a.) pili
b.) plasmid
c.) flagella
d.) capsule
12.) Antibiotics such as penicillin target which bacterial structure?
a.) capsid
b.) cell wall
c.) flagella
d.) ribosome
13.) What is the role of the bones in the ear (ossicles)?
a.) They deliver sound vibrations to the middle ear.
b.) They help maintain balance.
c.) They transfer nerve impulses to the brain to be interpreted.
d.) They collect and direct sound to the tympanic membrane.
14.) Structure 9 in the diagram is the
a.) vestibule
b.) semicircular canals
c.) cochlea
d.) cochlear nerve
15.) Which of the following shows the correct order of how sound travels through the ear?
a.) pinna, tympanic membrane, stapes, cochlea, cochlear nerve
b.) auditory canal, stapes, incus, cochlea, vestibular nerve
c.) pinna, tympanic membrane, vestibule, Eustachian tube
d.) tympanic membrane, incus, malleus, semicircular canal
17.) Which of the following is considered a medical intervention for hearing loss?
a.) sign language
b.) cochlear implant
c.) hearing aid
d.) all of these
18.) During a Rinne test, what does it mean when a patient can hear the tuning fork longer through bone conduction than through air conduction?
a.) conductive hearing loss
b.) sensorineural hearing loss
c.) complete hearing loss
d.) a problem with the brain's ability to process sound
19.) Which of the following is true about cochlear implant technology?
a.) The entire deaf community supports cochlear implants as a treatment for hearing loss.
b.) Cochlear implants are effective for everyone and have predictable results.
c.) A cochlear implant is an option for treating profound hearing loss.
d.) A cochlear implant is necessary for someone with hearing loss to function in mainstream society.
20.) Which of the following correctly describes vaccines?
a.) They are not considered medical interventions.
b.) They prime the innate immune system, which will then produce antibodies against the pathogen.
c.) They activate the adaptive immune response against an antigen so that it will "remember" the pathogen.
d.) They cause a mutation in the vaccinated person that makes them immune to the pathogen.
21.) Which of the following correctly shows the order of producing recombinant DNA?
I. Restriction enzyme recognizes specific nucleotide sequences on the plasmid.
II. Sticky ends of foreign DNA line up with sticky ends on the plasmid DNA.
III. Plasmid DNA is attached to foreign DNA by DNA ligase, an enzyme that "glues" DNA fragments together.
IV. Restriction enzyme cuts sugar-phosphate backbone on plasmid DNA, opening plasmid.
a.) I, IV, II, III
b.) I, II, III, IV
c.) III, I, IV, II
d.) IV, I, II, III
22.) Trudy is working as an ophthalmologist and wants to know whether chronic eye infections are associated with vision loss. She decides to follow two groups of her patients: those who get chronic eye infections and those who do not. She tracks patient data for 10 years to see if the patients in each group experience vision loss. This is an example of:
a.) case-control study
b.) cohort study
c.) retrospective study
d.) need more information to decide
