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WorksheetsMI 1.1.5 ELISA
Total questions: 16
Worksheet time: 17mins
What is the final dilution for Tube 3?
201
1/400
80001
1600001
What is the tube dilution for Tube 3?
201
1/400
80001
1600001
If 1 mL sample is put into tube #1 that has 24 ml diluent, what is the tube dilution? What is the final dilution?
Tube 1/25
Final 1/50
Tube 1/50
Final 1/25
What is the tube dilution?
10050
201
15050
31
4.) During the ELISA test, the secondary antibody was attached to an enzyme. What happens when that enzyme becomes attached to the substrate?
a.) The enzyme and the substrate reacted with the primary antibody and caused a color change.
b.) The enzyme and the substrate work worked together to destroy the antigen, resulting in a color change.
c.) The enzyme caused the substrate to change, resulting in a color change.
d.) The substrate caused the enzyme to change, resulting in a color change.
5.) In what order should you add the reagents to perform an ELISA test?
a.) Primary antibody, antigen sample, enzyme-linked secondary antibody, substrate
b.) Antigen sample, primary antibody, enzyme-linked secondary antibody, substrate
c.) Substrate, enzyme-linked secondary antibody, antigen samples
d.) Primary antibody, enzyme-linked secondary antibody, antigen samples, substrate
6.) ________ have surface _________ where _________ attach.
a.) antibodies, pathogens, antigens
b.) antigens, antibodies, pathogens
c.) pathogens, antibodies, antigens
d.) pathogens, antigens, antibodies
Meningitis is the inflammation of what in the brain?
Meninges
It involves the brain?
Spinal cord
Im not sure
Which of the following cannot be determined by an ELISA test?
Concentration of antigens
Presence of disease
Genetic sequence of a pathogen
Order of infection
After adding the secondary antibody and enzyme complex to the ELISA, what is added next (after washing) to create the color change?
substrate
primary antibody
enzyme #2
antigen
Which of the following is NOT a symptom of meningitis infection?
Fever
Lethargy
Stiff Neck
Rash with scabs
Put the steps of an ELISA assay in order
make serial dilutions of antigen of interest for comparison
add samples to be tested
add primary Ab to all wells, let develop then wash
add secondary Ab with attached enzyme to all wells, develop and then wash
add TMB substrate to all wells
