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MI 1.1.5 ELISA

Total questions: 16

Worksheet time: 17mins

Name
Class
Date
1.

What is the final dilution for Tube 3?

a)

120\frac{1}{20}  

b)

1/400

c)

18000\frac{1}{8000}  

d)

1160000\frac{1}{160000}  

2.

What is the tube dilution for Tube 3?

a)

120\frac{1}{20}  

b)

1/400

c)

18000\frac{1}{8000}  

d)

1160000\frac{1}{160000}  

3.

If 1 mL sample is put into tube #1 that has 24 ml diluent, what is the tube dilution? What is the final dilution?

a)

Tube 1/25

b)

Final 1/50

c)

Tube 1/50

d)

Final 1/25

4.
What would be the tube dilution?
a)
1/10
b)
2/10
c)
1/100
d)
1/1000
5.
What would be the final dilution?
a)
1/10
b)
1/100
c)
1/1000
d)
1/10000
6.

What is the tube dilution?

a)

50100\frac{50}{100}  

b)

120\frac{1}{20}  

c)

50150\frac{50}{150}  

d)

13\frac{1}{3}  

7.

4.) During the ELISA test, the secondary antibody was attached to an enzyme. What happens when that enzyme becomes attached to the substrate?

a)

a.) The enzyme and the substrate reacted with the primary antibody and caused a color change.

b)

b.) The enzyme and the substrate work worked together to destroy the antigen, resulting in a color change.

c)

c.) The enzyme caused the substrate to change, resulting in a color change.

d)

d.) The substrate caused the enzyme to change, resulting in a color change.

8.

5.) In what order should you add the reagents to perform an ELISA test?

a)

a.) Primary antibody, antigen sample, enzyme-linked secondary antibody, substrate

b)

b.) Antigen sample, primary antibody, enzyme-linked secondary antibody, substrate

c)

c.) Substrate, enzyme-linked secondary antibody, antigen samples

d)

d.) Primary antibody, enzyme-linked secondary antibody, antigen samples, substrate

9.

6.) ________ have surface _________ where _________ attach.

a)

a.) antibodies, pathogens, antigens

b)

b.) antigens, antibodies, pathogens

c)

c.) pathogens, antibodies, antigens

d)

d.) pathogens, antigens, antibodies

10.

Meningitis is the inflammation of what in the brain?

a)

Meninges

b)

It involves the brain?

c)

Spinal cord

d)

Im not sure

11.
A sample of spinal fluid is taken from a college student suspected to have contracted spinal meningitis.Which diagnostic test will confirm the diagnosis fastest?
a)
Culturing the spinal fluid and testing for bacterial colony growth in a Petri dish.
b)
An ELISA Assay to determine the presence of antigens or antibodies.
c)
DNA sequencing
d)
Protein Chromatography
12.

Which of the following cannot be determined by an ELISA test?

a)

Concentration of antigens

b)

Presence of disease

c)

Genetic sequence of a pathogen

d)

Order of infection

13.
What does ELISA stand for?
a)
E coli Immune System Antigen
b)
Enzyme-linked Immunosorbent Assay
c)
Enzyme Leuko-immune Standard Assessment
d)
Estimated Lymph Integrated System Assessment
14.

After adding the secondary antibody and enzyme complex to the ELISA, what is added next (after washing) to create the color change?

a)

substrate

b)

primary antibody

c)

enzyme #2

d)

antigen

15.

Which of the following is NOT a symptom of meningitis infection?

a)

Fever

b)

Lethargy

c)

Stiff Neck

d)

Rash with scabs

16.

Put the steps of an ELISA assay in order

a)

make serial dilutions of antigen of interest for comparison

b)

add samples to be tested

c)

add primary Ab to all wells, let develop then wash

d)

add secondary Ab with attached enzyme to all wells, develop and then wash

e)

add TMB substrate to all wells

1)
2)
3)
4)
5)