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WorksheetsProtein purification
Total questions: 15
Worksheet time: 8mins
●Protein purification refers to the:
Purification of different cellular components
The process of converting proteins into their primary structure
The process of separating and isolating a specific protein from a complex mixture
The process of denaturing proteins.
●Protein separation techniques are often based on the following properties except
Solubility of protein
Viscosity of protein
Charge of the protein
Specific binding affinity of the protein
●In which of the following separation method where proteins are separated based on their net charge
Affinity chromatography
Ion exchange chromatography
Dialysis
Gel filtration chromatography
●In gel filtration chromatography, the separation of proteins is based on their
Size and net charge
Size and shape
Size and specific affinity
Shape and net charge
●The use of insulin hormone to purify its receptor is an example of
Ion exchange chromatography
Affinity chromatography
Gel filtration chromatography
none of the above
In anion exchange chromatography
The column contains negatively charged beads where positively charged proteins binds
The column contains positively charged beads where negatively charged proteins bind
The column contains both positive and negatively charged beads where proteins bind depending on their net charge
All of these
The best method to hydrolyzed tryptophan is
Acid hydrolysis
Base hydrolysis
Salting out
Enzymatic hydrolysis
●For the study of a protein in detail, an effort is usually made to first,
Conjugate the protein to a known molecule
Determination of amino acid composition
Determination of amino acid sequence
Purify the protein
What is the primary purpose of using an internal standard in an HPLC method?
To increase the separation efficiency of the HPLC column
To enhance the sensitivity of the HPLC detector.
To monitor and correct for variations in sample preparation, injection volume, and detector response.
To decrease the retention times of analytes on the HPLC column.
What is the primary benefit of using pre-column derivatization in HPLC for amino acid analysis?
It reduces the need for sample preparation.
It reduces the separation of amino acids on the HPLC column.
It allows for direct detection of native amino acids.
It enhances the sensitivity and selectivity of detection.
●Phenylisothiocyanate (PITC ) AA derivatives can be detected through:
UV-visible spectroscopy
visible light detection
By fluorescent detector
none of the above
●The time taken by the analyte after sample injection to reach the detector is called _________
Dead time
Solute migration rate
Adjusted retention time
Retention time
In a chromatographic separation, which of the following is most appropriate for the qualitative analysis of a substance?
Area of the component peak
retention time
dead time
capacity factor
In which chromatography stationary phase is more polar than the mobile phase?
ion exchange chromatography
Revered phase chromatography
normal phase chromatography
size exclusion chromatography
Which of the following statements is true when comparing internal standards and external standards in HPLC for amino acid detection?
Internal standards are never used in amino acid analysis.
External standards provide a direct measurement of amino acid concentrations.
Internal standards are added to the sample, while external standards are injected separately.
External standards are always preferred over internal standards for accuracy.
