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Topic 1: Recovery Processes (ECH4201)

Total questions: 50

Worksheet time: 30mins

Name
Class
Date
1.

The various steps of Downstream Processing includes

a)

Separation and Cell disruption

b)

Extraction and Isolation

c)

Purification and Drying

d)

All of the above

2.

Which one of the following product in pharmaceutical industry produce through downstream processing

a)

Antibiotics

b)

Hormones

c)

Antibodies

d)

All of the above

3.

Which of the following is upstream processing?

a)

Product recovery

b)

Product purification

c)

Media formulation

d)

Cell lysis

4.

Which of the following is downstream processing

a)

Product recovery

b)

Isolation of microorganism

c)

Media formulation

d)

Design of reactor

5.

What factors affect the selection of downstream processing method?

a)

Capital expenses, processing expenses

b)

Probable yield, product quality

c)

Availability of technical skill

d)

All of the above

6.

What types of information you are able to get from Process Flow Diagram (PFD)?

a)

List of major equipment

b)

Process Flow & Utilities Stream

c)

Basic Control Loop

d)

All items above

7.

The various steps of Downstream Processing includes

a)

Separation and Cell disruption

b)

Extraction and Isolation

c)

Purification and Drying

d)

All of the above

8.

Disruption of cell is generally achieved by

a)

Mechanically

b)

Lysis

c)

Drying

d)

All of the above

9.

The most frequent approaches of drying are

a)

Vacuum drying

b)

Spray drying

c)

Freeze drying

d)

All of the above

10.

The process of extraction is achieved by

a)

Liquid-liquid extraction

b)

Whole broth (medium + cells) extraction

c)

Aqueous multiphase extraction

d)

All of the above

11.

Identify the correct sequence during the industrial production of substances.

a)

Inoculation, screening, fermentation, downstream processing, removal of waste

b)

Screening, Inoculation, fermentation, downstream processing, removal of waste

c)

Fermentation, screening, inoculation, removal of waste, downstream processing

d)

Fermentation, inoculation, inoculation, removal of waste, downstream processing

12.

Which are most closely related to biotechnology?

a)

medicine and agriculture

b)

construction and engineering

c)

water treatment and electricity generation

d)

communication and information technology

13.

Which area of biotechnology would most likely create ethical issues within human society?

a)

insulin production by bacteria

b)

organ cloning for use in transplants

c)

genetic engineering to improve agricultural yields

d)

DNA and forensic testing of crime scene evidence

14.

Which is most important when investigating ethical issues in biotechnology?

a)

cost of the technology

b)

advantage of the technology

c)

public opinion of the technology

d)

benefits of the technology outweighing the harm

15.

Which was most likely the earliest way biotechnology was studied by humans?

a)

finding evidence of crimes

b)

making new medicines to cure sicknesses

c)

discovering the cause of hereditary diseases

d)

breeding better varieties of crops and livestock

16.

Which is a genetic engineering advancement directly related to a career in biotechnology?

a)

improving solar energy collection

b)

transporting textiles at higher rates

c)

creating crop foods that resist insect pests

d)

repairing historical sites with quality materials

17.

Which would be reduced as a result of the development of pest-resistant crops?

a)

use of organic fertilizers

b)

use of chemical insecticides

c)

practice of crop rotation techniques

d)

practice of hydroponic farming techniques

18.
The process of changing the genetic material of a living organism 
a)
Genetic Modification
b)
Taxonomy
c)
Adapting
d)
Bioremediation
19.

This is the practice of producing a genetically identical cell or organism

a)

Cloning

b)

Gel Electrophoresis

c)

Genetic Engineering

d)

DNA Fingerprinting

20.
What is recombinant DNA?
a)
Adding DNA from one organism into the DNA of another
b)
DNA which has been changed over generations by natural selection
c)
DNA that causes genetic disorders
d)
DNA that has been sequenced
21.
Another name for GMO (Genetically Modified Organsim) is ____________.
a)
Transgenic
b)
Transplanted
c)
Transducted
d)
Plasmid
22.

The process that has been used for centruries to create purebred animals such as black labs is called

a)

recombinant DNA

b)

cloning

c)

selective breeding

23.

The insertion of a gene of interest into a bacterial DNA loop is called

a)

recombinant DNA

b)

insertion technique

c)

mutation propagation

24.

Selective breeding is...

a)

a process to create an organism with desired traits from parents with those desired traits

b)

natural method of producing offspring with desired traits

c)

creating a new species

d)

making organisms reproduce during times of stress

25.

An example of selective breeding is

a)

a dog breeder mating 2 dogs to make more dogs

b)

a farmer grows bees to pollinate the plants on his farm

c)

a person pollinates 2 white rose plants to only create white roses

26.
Genetic engineering has been used to produce
a)
medicines.
b)
food animals.
c)
improved crops.
d)
all of these.
27.

What are genetically modified (GM) organisms and GM foods?

a)

Foods or organisms with better taste

b)

Foods or organisms grown in the USA, only

c)

Foods or organisms with DNA modified in a way that doesn't occur naturally

d)

Food and organisms that have been injected with hormones

28.

Which of the following are ways that Biotechnology can be used?

a)

Diagnosis of disease

b)

Gene therapy

c)

Production of pharmaceuticals (medicine)

d)

DNA profiling

e)

Agriculture - creating GMOS (Genetically modified organisms)

29.

Which is a concern of scientists when genetically modifying plants?

a)

Plants will pass on diseases to animals.

b)

There will be a decrease in biodiversity.

c)

Plants will have a longer growing season.

d)

There will be a decrease in revenue for pesticide manufacturers.

30.

Which best describes a controversial issue associated with the use of genetically modified crops?

a)

the use of genetically modified crops to increase potential yield

b)

the short term use of genetically modified crops in famine stricken countries

c)

the development of genetically modified crops which are more nutrient dense

d)

the long term effects which may arise from the use of genetically modified crops

31.

Which of these is NOT an example of Biotechnology?

a)

using a car for transportation

b)

creating oil eating bacteria to clean up oceans

c)

cloning of organs

d)

a vaccine

32.

Breeding two dissimilar organisms to get the best characteristics of both is called

a)

hybridization

b)

selective breeding

c)

transgenic organisms

d)

recombinant breeding

33.

The purpose of the Human Genome Project was to

a)

Map out all of the genes in human DNA

b)

Combine human DNA with other organisms

c)

Create superhumans

34.

Antibiotics are used to treat viral infections

a)

true

b)

false - Vaccines are used to treat viral infections

35.

Why did scientists edit the genes of bacteria to produce insulin?

a)

Because the bacteria were diabetic

b)

Because bacteria have simple DNA that is easier to manipulate, making it easy to produce insulin in a lab

c)

Because bacteria's complex DNA was perfect for insulin production

d)

Because insulin has no use outside of a lab setting

36.
Which field would most likely NOT incorporate  biotechnology?
a)
Agriculture 
b)
Genetics 
c)
Medicine
d)
Mining 
37.
Which agricultural benefit can biotechnology  provide?
a)
developing plants that are less dependent on the sun for energy
b)
developing plants that are less sensitive to variations in environmental temperatures
c)
developing plants with inheritable traits unrelated to the genetic material they contain
d)
developing plants that will not die
38.
______________uses bacteria to clean up our surrounding.
a)
Genome
b)
Bioremediation 
c)
Genetic Modification 
d)
Crossbreeding 
39.

What does Bt stand for?

a)

Bacteria Transformation

b)

Bacillus Thuringiensis

c)

Blue Tiger

40.
1. Which of the following is a product made by biotechnology
a)
Bread
b)
Cheese
c)
Penicillin
d)
All of these
41.
One example of biotechnology is the process of making fuel from
a)
Oil Spill
b)
Corn
c)
Stem Cells
d)
Cheese
42.
How long have people been using processes that are considered forms of biotechnology?
a)
Thousands of Years
b)
1000 Years
c)
150 Years
d)
20 Years
43.
The word biotechnology has been in use since the
a)
1700s
b)
1800s
c)
1900s
d)
2000s
44.
What is DNA?
a)
The smallest unit of living organisms
b)
The basic genetic material found in cells
c)
A protein that accelerates the rate of chemical reactions
d)
A container used for bioprocessing
45.
Amino acids are the building blocks of:
a)
Fats.
b)
.Carbohydrates.
c)
.Proteins.
d)
.Neutrons.
46.
Which is a way the agricultural biotechnology industry could have a positive impact on the environment? 
a)
by producing crops that are virus resistant
b)
by reducing the need for countries to import food 
c)
by making robots to replace large farm machines
d)
by increasing the use of wind farms that produce electricity 
47.

Bacteria cells can be used to produce insulin for the treatment of diabetes in humans. Which best explains how the bacteria cells are able to produce insulin for humans?

a)

The bacteria have been grown on a petri dish containing insulin.

b)

The bacteria have been genetically engineered to contain the DNA

needed to produce insulin.

c)

The bacteria have been exposed to radioactivity to create mutant

strains capable of producing insulin.

d)

The bacteria have been grown on a petri dish containing sugar; the

bacteria produce insulin in response to the sugar.

48.

The recent pandemics of Covid-19 saw the development of new vaccines. Which area of biotechnology is this related to?

a)

Green biotechnology

b)

Blue biotechnology

c)

Red biotechnology

d)

Dark biotechnology

49.

Anthrax and botulism toxin used as bioterrorism and biowar is often associated with...

a)

Black biotechnology

b)

Pink biotechnology

c)

Purple biotechnology

d)

Dark biotechnology

50.

This area of biotechnology emphasizes on agriculture that involves creating new plant varieties of agricultural interest, biopesticides, and biofertilizers.

a)

Blue biotechnology

b)

Green biotechnology

c)

Yellow biotechnology

d)

Red biotechnology

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