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2.2.6 - A Family Affair

Total questions: 13

Worksheet time: 9mins

Name
Class
Date
1.

Which of the following terms represents a molecule that acts as molecular scissors that can cut DNA in specific locations, called recognition sites.

a)

Polymerase Chain Reaction

b)

Gel Electrophoresis

c)

Restriction Enzyme

d)

Restriction Digestion

2.

DNA is located in the (a)   of human cells.

3.

DNA has a ______ charge.

a)

positive

b)

neutral

c)

negative

4.
What is the purpose of gel electrophoresis?
a)
helps cut DNA
b)
count the genes in DNA
c)
separates DNA based on size
d)
allows for an exact replicated organism
5.
Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?
a)
DNA is negatively charged so attracted to the positive end of the unit
b)
DNA is positively charged to attracted to the negative end of the unit
c)
the agarose gel in negatively charged
d)
the agarose gel is positively charged
6.
In gel electrophoresis, the largest DNA fragment will appear
a)
closest to the starting wells
b)
farthest from the starting wells
c)
three quarters away from the starting wells
d)
it depends on how many fragments there are
7.
Which is the primary purpose of using restriction enzymes in gel electrophoresis?
a)
It allows the strands of DNA to be cut into various lengths for testing
b)
It restricts the number of base pairs that can be tested in a sample
c)
It makes the testing simpler by moving the strands into the gel faster
d)
It charges the DNA strands
8.

To create a DNA profile, the DNA must be copied multiple times. Which of the following is the process by which the DNA is copied?

a)

DNA extraction

b)

gel electrophoresis

c)

restriction enzyme digest

d)

polymerase chain reaction (PCR)

9.

The hole that the DNA is injected into

a)

Power Source

b)

Lane

c)

Well

d)

Tank

e)

Marker / DNA Ladder

10.
a)

micropipette

b)

transfer pipette

c)

pipette pump

d)

serological pipette

11.
When using a micropipettor, if you push to the second stop to fill it you will get ____________
a)
the correct amount of solution
b)
too much solution
c)
too little solution
d)
either too much or too little solution
12.

Always change out the micropipette tip when changing reagents.

a)

true

b)

false

13.

This is a p20 micropipette.

How many microliters will this micropipette take up?

a)

500 uL

b)

50 uL

c)

5.0 uL

d)

0.5 uL