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WorksheetsMicroscope
Total questions: 20
Worksheet time: 10mins
Label the part of the microscope. What is part A?
eyepiece lens
eyepiece tube
turret
objective lens
Label the part of the microscope. What is part D?
body
foot
objective lens
turret
Moves stage up and down for focusing; locates the specimen
Fine Focus Knob
Large Focus Knob
Course Focus Knob
Small Focus Knob
Which objective should be used to first find an image?
Low power
Medium power
High power
Which part of the microscope should you use to sharpen the image?
Diaphragm
Coarse adjustment
Stage
Fine adjustment
We begin to look at our specimen using which combination?
high power and fine adjustment knob
high power and coarse adjustment knob
low power and coarse adjustment knob
low power and fine adjustment knob
Which scenario is correct?
Focusing a slide under high power with the coarse adjustment knob.
Focusing a slide under low power with the coarse adjustment knob
Carrying the microscope by the base and body tube
Focusing a slide by moving the diaphragm
Typical objective lenses come in magnifications of ___
6x, 15x, 60x
60x, 150x, 600x
4x, 10x, 40x
40x, 100x, 400x
Instead of light, a TEM, STM, and SEM utilize ____________ to create an image
neutrons
radiation
probe
electron
TEM creates images based on electrons that are ________________.
reflected
absorbed
frozen
heated
True or False? SEM images look 3D and are colorful.
True
False
Which electron microscope provides details of a cell's internal structures?
Scanning electron microscope (SEM)
Transmission electron microscope (TEM)
Light microscope
Dissecting microscope
Which microscope would you use to see a ed bug in 3D, with a magnification of about 500,000?
Compound microscope
Scanning electron microscope (SEM)
Transmission electron microscope (TEM)
Fluorescent microscope
Choose false statement about fluorescence microscope
Uses UV light
Cells may be stained with fluorescent dyes
Diagnostic technique involved immunofluorescence or fluorescent-antibody technique
Very clean 3D image of cells can be viewed
Choose false statement about scanning-probe microscopy
Uses metal or diamond probe inserted into the specimen
Used to map atomic and molecular shapes, to characterize magnetic and chemical properties and to determine temperature variations inside cells
No special preparation of specimen is required
Expose the specimen to damaging high energy radiation
Choose false statement about confocal microscopy
Uses UV light
Can be used to evaluate cellular physiology by monitoring the distributions and concentrations of intracellular substances
Allows obtaining 3D images of the sample with good resolution
Can overcome limitation of fluorescence microscope
Choose false statement
No stain required for phase contrast microscopy
In darkfield illumination, dark objects are visible against bright background
In lightfield illumination, dark objects are visible against bright background
Immersion oil is used to resolve the power of 100X objective lense
