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BIOL309 Exam 2 Lecture Review: Chapter 15 Transcription

Total questions: 19

Worksheet time: 23mins

Name
Class
Date
1.

3 major types of RNA in Transcription

a)

mRNA, siRNA, tRNA

b)

snRNA, mRNA, uRNA

c)

rRNA, siRNA, tRNA, snRNA

d)

mRNA, tRNA, rRNA

2.

Compare: DNA Replication and Transcription

4 lines
3.

Differentiate: DNA Replication and Transcription

4 lines
4.

What does RNA polymerase require?

a)

Phosphate ions and uracil

b)

Calcium ions and dNTPs

c)

Manganese ions and UTP

d)

Magnesium ions and rNTPs

5.

How does RNA Pol add ribonucleotides?

a)

3’ OH and build RNA 5’ to 3’

b)

5’ OH and build RNA 3’ to 5’

c)

5’ OH and build RNA 5’ to 3’

d)

3’ OH and build RNA 3’ to 5’

6.

Function: Sigma factor in RNA Pol

4 lines
7.

RNA Poly in eukaryotes

a)

RNA Pol V

b)

RNA Pol III

c)

RNA Pol IV

d)

RNA Pol II

e)

RNA Pol I

8.

Function: Pol I

4 lines
9.

Function: Pol II

4 lines
10.

Function: Pol III

4 lines
11.

Briefly Describe: Initiation, Elongation, and Termination similarity in Prokaryotes and Eukaryotes (according to lecture slides)

4 lines
12.

Which strand will be expressed by the RNA?

a)

RNA

b)

Template

c)

Nontemplate

d)

Coding

13.

Transcription begins with a…

(a)  

14.

E. Coli generally keeps about __ bp of DNA unwound

a)

15

b)

16

c)

18

d)

17

15.

Topological problems are solved by (a)   , which move along DNA

16.

Topological strain is seen in which direction?

a)

Downstream

b)

Upstream

c)

Both

17.

Why is it okay for transcription to have a higher error rate than DNA replication?

a)

DNA is complex, we need energy to fix RNA

b)

Make a lot more RNA

c)

RNA is strict and requires full functionality

d)

RNA does not last long

e)

Nonfunctional RNA is harmless

18.

Simple General Steps of Transcription

4 lines
19.

Where does much of the regulation of protein levels occur?

a)

Initiation steps

b)

Termination steps

c)

Promoter-Binding sequence

d)

Elongation steps