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PCR Review

Total questions: 10

Worksheet time: 6mins

Name
Class
Date
1.

What are some of the functions of PCR

a)

Confirm Cancer

b)

Diagnostics

c)

Identify mutations

d)

Observe mutations

2.

Kary (a)   discovered PCR in 1984

3.

What is not found in PCR

a)

Taq polymerase

b)

Nucleotides

c)

Polymerase

d)

Cofactor

4.

Which one isn't a part of the PCR process?

a)

Denaturing

b)

Copying

c)

Annealing

d)

Extension

5.

What is denaturation?

a)

Proteins binding together

b)

Proteins gaining structure

c)

Proteins outside of their optimum range

d)

Proteins losing their shape

6.

What does PCR stand for?

(a)  

7.

What are primers?

a)

Short DNA sequences that provide a starting point for DNA synthesis

b)

Long DNA sequences that provide a starting point for DNA synthesis

c)

A protein that binds to the DNA to mark the starting point

d)

Inhibitors

8.

The polymerase enzyme (a)   a new DNA strand

9.

How is reverse transcription different from conventional PCR?

a)
Reverse transcription creates DNA from RNA, while conventional PCR amplifies RNA directly.
b)
Reverse transcription creates cDNA from RNA, while conventional PCR amplifies DNA directly.
c)
Reverse transcription creates RNA from DNA, while conventional PCR amplifies RNA directly.
d)
Reverse transcription creates RNA from DNA, while conventional PCR amplifies DNA directly.
10.

Why is PCR so significant in the field of molecular biology?

a)
It allows for the amplification of specific DNA sequences
b)
It is used to study the behavior of electrons
c)
It helps in predicting weather patterns
d)
It is used for cooking food