Worksheets1.1.6. Electrophoresis Quiz
Total questions: 18
Worksheet time: 9mins
Name
Class
Date
1.
If you are making a 30 mL gel, how much concentrated buffer should you add? I want the answer in MICROLITERS, since you'll be using the micropipetter. Type the answer with NO unit symbol after it.
(a)
2.
If you are making a 0.8% 30 mL gel, how much agarose powder should you add? The answer will be in grams. Type the answer with NO unit symbol after it.
(a)
3.
Approximately how long will you need to microwave the mixture?
a)
10 seconds
b)
1 minute
c)
2 minutes
d)
5 minutes
4.
How many wells should the combs we use have?
a)
6
b)
8
c)
10
d)
12
5.
If we're making a 30 mL gel, how many microliters of DILUTED SYBR Safe should be added? Type the answer without a unit.
(a)
6.
When should you pour the mixture into the gel tray
a)
As soon as it comes out of the microwave
b)
As soon as it reaches 37 degrees C
c)
As soon as it's cool enough to hold in the palm of your hand
d)
Once it reaches room temperature
7.
When is the SYBR Safe added?
a)
before microwaving mixture
b)
after microwaving mixture & before pouring it
c)
after pouring mixture into gel tray
d)
when loading DNA into gel
8.
If using your gel the next day, how should it be stored?
a)
on the counter at room temperature
b)
in the incubator at body temperature
c)
in the refrigerator
d)
in the electrophoresis apparatus at room temperature
9.
How many mL of running buffer will fill our M12 chamber? Type the answer without a unit.
(a)
10.
To make our running buffer, how many mL of concentrated buffer should we add? Type the answer without a unit.
(a)
11.
When loading DNA, we load it from
a)
top to bottom
b)
bottom to top
c)
left to right
d)
right to left
12.
What should be on top of your gel chamber as it runs?
a)
the foil
b)
the plastic baggie
c)
the empty DNA strip
d)
the instructional handout
13.
How do we know electricity is flowing?
a)
there's a color change
b)
bubbles form at ends of chamber
c)
the gel starts to glow
d)
the buffer starts to boil
14.
DNA is _____________ so is loaded at the ______________ end and flows toward the _____________ end
a)
positive, positive, negative
b)
positive, negative, positive
c)
negative, negative, positive
d)
negative, positive, negative
15.
Our gel will run at a voltage of ______ for approximately ________. Select both.
a)
125 V
b)
40 minutes
c)
75 V
d)
100 V
e)
150 V
16.
What must be done when using the transilluminator?
a)
remove gel from baggie
b)
turn off lights
c)
add SYBR safe to tray
d)
add ethidium bromide to tray
17.
The overall purposes of gel electrophoresis ARE (select both).
a)
cut DNA pieces
b)
separate DNA pieces
c)
amplify DNA sample
d)
denature DNA sample
e)
make DNA visible
18.
When we view the gel & compare lanes, we're trying to find
a)
Which lane has the MOST RFLPs
b)
Which lane matches the standards
c)
Which lanes match one another
d)
Which lanes have DNA in them
100 %
