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PCR mini quiz

Total questions: 10

Worksheet time: 10mins

Name
Class
Date
1.

The process of PCR that seperates the double stranded DNA template at a high temperature,

(a)  

2.

What bacteria is the Taq DNA polymerase from?

(a)  

3.

What is the range of base nucleotides in a primer used in PCR?

use the format "___ - ___"

(a)  

4.

Provides a suitable chemical environment for DNA polymerase activity.

(a)  

5.

Type of PCR

More than one set or primers are used to amplify multiple sequences at the same time

(a)  

6.

Application of PCR

Desired gene is isolated and amplified via PCR. It is then inserted into a vector (dsDNA carrier) that can be injected into a bacteria or cell that will express the gene or transfer the gene to the target organism.

(a)  

7.

Type of PCR

Amplicons produced is quantified real-time, as the sample goes through the cycles
*acronym ang gamitin pangsagot.

(a)  

8.

PCR Master Mix

DNA to be amplified.

(a)  

9.

Stage of pcr that has a spontaneous binding of primers to target DNA as temperature decreases.

(a)  

10.

DNA copies produced are called (a)   .