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Cell Culture Technologies-II M.Sc. Biotechnology

Total questions: 10

Worksheet time: 5mins

Name
Class
Date
1.

What is a cell line?

a)

Transformed cells

b)

Multilayer culture

c)

Sub culturing of primary culture

d)

Multiple growth of cells

2.

Cross-contamination is

a)

Not to much of a problem and never happens

b)

The result of working with multiple cells at one time

c)

Can be controlled with antibiotics

d)

Cannot really happen if you follow good protocols

3.

In the secondary culture, cells are obtained from _______________________

a)

Primary culture

b)

Organ culture

c)

Phenotypic culture

d)

None of the above

4.

WHICH IS ESSENTIAL FOR A CELL'S GROWTH?

a)

ASEPTIC LAB

b)

SKILLED TECHNICIAN

c)

GROWTH MEDIA

d)

ALL THE ABOVE

5.

What are the units used to express cell concentration in the hemocytometer method?

a)

cells/µm

b)

cells/mL

c)

cells/µL

d)

cells/mg

6.
The longest phase of the cell cycle 
a)
prophase
b)
interphase
c)
metaphase
d)
mitosis
7.

During which cycle can you identify chromosomes

a)

G1 phase

b)

G2 phase

c)

S phase

d)

M phase

8.

It refers to the isolation of cells from an animal or plant and their subsequent growth in a favorable artificial environment.

a)

Plant Extraction

b)

Polymerase Chain Reaction

c)

Cell Culture

d)

DNA Extraction

9.

Which one of the following is the most appropriate disinfectant for cleaning the work area (Laminar Flow Cabinet)?

a)

Saline

b)

Water

c)

70% ethanol

d)

9% ethanol

10.

Who is known as the father of Cell Culture Techniques?

a)

Ross Harrison

b)

Alexis Carrel

c)

Harry Eagle

d)

Renatto Dulbecco