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DNA-Serology Review

Total questions: 57

Worksheet time: 43mins

Name
Class
Date
1.

What is the main reason for packaging items suspected of containing DNA evidence in paper bags instead of plastic?

a)

Plastic bags are not readily available at crime scenes

b)

Paper bags are easier to handle

c)

Plastic retains moisture which can destroy DNA evidence

d)

Paper bags are cheaper

2.

What are some sources of DNA that can be found in a crime scene?

a)

Sweat and dandruff

b)

Saliva and mucus

c)

All of the above

d)

Ear wax and blood

3.

True or False: Red blood cells DO NOT contain a nucleus, therefore do not have DNA.

a)

True

b)

False

4.
Which is the primary purpose of using restriction enzymes in gel electrophoresis?
a)
It allows the strands of DNA to be cut into various lengths for testing
b)
It restricts the number of base pairs that can be tested in a sample
c)
It makes the testing simpler by moving the strands into the gel faster
d)
It charges the DNA strands
5.

What is the technology that makes multiple copies of a specific DNA segment?

a)

Replication

b)

Transcription

c)

Translation

d)

Polymerase Chain Reaction

6.

What is the name of the technique used to separate DNA fragments by size?

a)

Chromatography

b)

Electrophoresis

c)

Centrifugation

d)

Distillation

7.
Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?
a)
DNA is negatively charged so attracted to the positive end of the unit
b)
DNA is positively charged to attracted to the negative end of the unit
c)
the agarose gel in negatively charged
d)
the agarose gel is positively charged
8.
In gel electrophoresis, the largest DNA fragment will appear
a)
closest to the starting wells
b)
farthest from the starting wells
c)
three quarters away from the starting wells
d)
it depends on how many fragments there are
9.

Whose DNA was found in the blood stain at the crime scene?

a)

Bob

b)

Sue

c)

John

d)

Lisa

10.

Which DNA strands move the fastest through the agarose gel when electricity is applied?

a)

long strands

b)

short strands

c)

medium strands

d)

They all move the same

11.

How many distinctive markers are used for DNA fingerprinting to distinguish between two people?

a)

10

b)

13

c)

15

d)

20

12.

There are multiple male suspects involved in a case. Which of the following analysis techniques would be most helpful in differentiating the men from one another ?

a)

Y-Marker Analysis

b)

SNP Analysis

c)

Amelogenin Gene Analysis

d)

Mitochondrial DNA Analysis

13.

The computerized database for DNA profiles that is maintained by the FBI

a)

IAFIS

b)

CODIS

c)

NIBIN

d)

IBIS

14.

DNA can be used for ...

a)

determining paternity

b)

identifying human remains

c)

identifying a suspect

d)

All of these

15.

Which suspect's DNA matches the DNA left at the crime scene?

a)

Suspect 1

b)

Suspect 2

c)

Suspect 3

d)

All are excluded

16.

How many pairs of chromosomes do humans have?

a)

46 pairs

b)

30 pairs

c)

20 pairs

d)

23 pairs

17.

What are the nitrogenous bases found in DNA?

a)

A, T, G, U

b)

A, T, G, C

c)

A, T, U, C

d)

A, G, C, U

18.

What is the process in which DNA makes a copy of itself?

a)

DNA Mutation

b)

DNA Transcription

c)

DNA Replication

d)

DNA Translation

19.

What is the shape of the DNA molecule?

a)

Spiral

b)

Double Helix

c)

Single Helix

d)

Circle

20.

Which enzyme is responsible for unzipping the DNA double helix during replication?

a)

Ligase

b)

Helicase

c)

Polymerase

d)

Nuclease

21.
In DNA, Adenine bonds with ______________.
a)
Guanine
b)
Thymine
c)
Cytosine
d)
Adenine
22.

Guanine bonds with ______________.

a)
Adenine
b)
Guanine
c)
Cytosine
d)
Thymine
23.
Why is DNA important?
a)
it is very small and very complicated
b)
it's in everything
c)

it serves as the blueprint for everything a living organism needs

d)
because we eat it every day for energy
24.

Which 2 molecules forms the sides and backbone of the DNA ladder?

a)
deoxyribose and adenine
b)
deoxyribose and a hydrogen bond
c)
sugar and the nucleus
d)
deoxyribose (sugar) and phosphate
25.
What does DNA stand for?
a)
Deoxyribonucleic acid
b)
Denitrogenous acid
c)
Deribonucleic acid
d)
Diribonucleic acid
26.

DNA in its tightly coiled form is referred to as

a)

chromosomes

b)

nitrogen bases

c)

genes

d)

nucleus

27.
What determines the code, or information, of a DNA molecule?
a)

the shape (structure) of the nitrogen bases

b)

the order (sequence) of the nitrogen bases

c)

the color of the nitrogen bases

d)

the frequency (number) of nitrogen bases

28.
The main goal of the Human Genome Project (HGP) was to:
a)

Identify the unique parts of each person's DNA.

b)

Identify genes responsible for human diseases.

c)

Identify genes responsible for human behavior.

d)

Determine the entire sequence of human DNA.

29.

DNA is found in all cells that have a _____________.

a)

chromosome

b)

nucleus

c)

CODIS

d)

napalm

30.

True or False: Red blood cells DO NOT contain a nucleus, therefore do not have DNA.

a)

True

b)

False

31.

Mitochondrial DNA is inherited from:

a)

Just mom

b)

both parents

32.

The name of the FBI's DNA database is:

a)

CODIS

b)

PCR

c)

RFLP

33.

Why is non-coding DNA the best for identifying individuals?

a)

It has to code for important proteins.

b)

It is not important for the cell's production of protein and thus can have different amounts for everyone.

c)

It is not important so it is not the best.

34.

Which process uses long fragments of repeating nitrogen bases to create a unique DNA fingerprint for an individual?

a)

RFLP

b)

STR

c)

PCR

d)

Recombinant DNA

35.

This process uses short repeating sections of DNA to identify an individual.

a)

Gel Electrophoresis

b)

PCR

c)

STR Analysis

d)

RFLP Analysis

36.

Biological evidence should be

a)

stored in air-tight containers

b)

frozen using liquid nitrogen

c)

packaged as quickly as possible

d)

put into breathable containers

37.
What does PCR stand for?
a)

Poly-ambiguous chain reaction

b)

Pentahedral corrosion remnant

c)

Polymerase chain reaction

d)

Peroxide corrosion remnant

38.
What is the purpose of PCR?
a)

To determine paternity

b)

To determine if a crime scene sample matches a suspect

c)

Generate a DNA profile

d)

Generate multiple copies of DNA

39.

This technique allows scientists to separate DNA fragments by size.

a)

PCR

b)

electrophoresis

c)

STR

d)

RFLP

40.
Which male is the father of this child?
a)

Male 1

b)

Male 2

c)

Neither could be the father

d)

Either could be the father

41.
Whose blood was found at the crime scene?
a)

John

b)

Lisa

c)

Bob

d)

Sure

42.

Who can be included as source of paternal DNA for this child?

a)

male #1

b)

male #2

c)

neither male

43.

What is the primary function of blood?

a)

To transport gases

b)

To provide defenses for the body

c)

To distribute nutrients

d)

All of the above

44.

What is the function of red blood cells (RBCs)?

a)

To produce antibodies

b)

To interact with clotting proteins

c)

To carry oxygen from the lungs to the body's tissues

d)

To fight off bacteria and viruses

45.

What is the function of white blood cells (WBCs)?

a)

To carry oxygen from the lungs to the body's tissues

b)

To destroy bacteria

c)

To interact with clotting proteins

d)

To carry nutrients

46.

What is the main function of platelets?

a)

To destroy bacteria

b)

To stop or prevent bleeding

c)

To produce antibodies

d)

To carry oxygen from the lungs to the body's tissues

47.

What is the primary function of plasma?

a)

To provide defenses for the body

b)

To distribute nutrients

c)

To transport gases

d)

To serve as the medium for blood cells

48.
What blood spatter pattern is seen in the picture?
a)

Transfer

b)

Passive

c)
Wipe
d)

Projected

49.
What type of blood spatter is typically created from a gunshot?
a)
Medium Velocity
b)
High Velocity
c)
Cast-Off
d)
Void
50.
The drop shown above would have more than likely been dropped from an angle of 
a)
90
b)
50
c)
30
d)
10
51.
Based upon this picture, what test was done to determine whether or not a stain was blood (the picture shows a positive and negative test). 
a)
Kastle-Meyer presumptive blood test
b)
Leukomalachite green presumptive blood test
c)
Luminol presumptive blood test
52.
What type of spatter creates the largest blood droplets?
a)
Low velocity impact
b)
Medium velocity impact
c)
High velocity impact
53.
What type of blood-spatter creates the smallest blood drops?
a)
Low velocity impact
b)
Medium velocity impact
c)
High velocity impact
54.
What type of evidence is blood type?
a)
Individual
b)
Class
c)
Trace
d)
Physical
55.
What blood stain pattern is created or formed by the force of gravity acting alone?
a)
Passive Drop
b)
Low Velocity Impact Spatter
c)
Flow Pattern
d)
Drip Pattern
56.

What type of blood spatter is shown here?

a)

transfer stains

b)

medium impact spatter

c)

high impact spatter

d)

arterial spray

57.

A blood droplet is 1.5cm long and .5cm wide. What is the angle of impact?

a)

21.5 deg

b)

19.5 deg

c)

8.86 deg

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