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QUALITY-TLC (READING MATERIALS)

Total questions: 10

Worksheet time: 5mins

Name
Class
Date
1.

What is the principle of thin layer chromatography (TLC)?

a)

Separation of compounds based on their weight

b)

Separation of compounds based on their odor

c)

Separation of compounds based on their color

d)

Separation of compounds based on differential migration in a stationary phase and mobile phase

2.

What is the stationary phase in thin layer chromatography (TLC)?

a)

Thin layer of adsorbent material on a solid support

b)

Glass or plastic plate

c)

Solvent carrying the sample compounds

d)

UV light, iodine vapor, chemical reagents

3.

What is the mobile phase in thin layer chromatography (TLC)?

a)

Thin layer of adsorbent material on a solid support

b)

Solvent or solvent mixture carrying the sample compounds

c)

Separation of compounds based on their odor

d)

Ratio of compound's migration distance to solvent front's distance

4.

How do compounds interact with the stationary phase in thin layer chromatography (TLC)?

a)

Based on their odor

b)

Based on their weight

c)

Based on polarity, size, charge, and other factors

d)

Based on their color

5.

What is the visualization method for compounds in thin layer chromatography (TLC)?

a)

UV light, iodine vapor, chemical reagents

b)

Separation of compounds based on their color

c)

Thin layer of adsorbent material on a solid support

d)

Solvent or solvent mixture carrying the sample compounds

6.

What is the parameter used to measure compound's migration in thin layer chromatography (TLC)?

a)

Separation of compounds based on their odor

b)

Rf (retention factor): Ratio of compound's migration distance to solvent front's distance

c)

Solvent or solvent mixture carrying the sample compounds

d)

Thin layer of adsorbent material on a solid support

7.

What are the purposes of thin layer chromatography (TLC)?

a)

Analyzing complex mixtures, Identifying specific compounds, Monitoring reaction progress, Determining purity of substances

b)

Separation of compounds based on their odor

c)

Separation of compounds based on their color

d)

Separation of compounds based on their weight

8.

What are the advantages of thin layer chromatography (TLC)?

a)

Simple and cost-effective technique, Quick results, Requires minimal equipment

b)

Might not provide high resolution compared to HPLC, Less quantitative compared to other techniques

c)

Thin layer of adsorbent material on a solid support

d)

Solvent or solvent mixture carrying the sample compounds

9.

What are the limitations of thin layer chromatography (TLC)?

a)

Thin layer of adsorbent material on a solid support

b)

Solvent or solvent mixture carrying the sample compounds

c)

Simple and cost-effective technique, Quick results, Requires minimal equipment

d)

Might not provide high resolution compared to HPLC, Less quantitative compared to other techniques

10.

What is the separation time for compounds in thin layer chromatography (TLC)?

a)

Solvent or solvent mixture carrying the sample compounds

b)

Requires minimal equipment

c)

Thin layer of adsorbent material on a solid support

d)

Relatively quick, typically 15 to 30 minutes