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WorksheetsPTC Lab Procedure and Purpose
Total questions: 11
Worksheet time: 6mins
What is the purpose of x-tract buffer in PCR (Polymerase Chain Reaction) procedure?
To amplify DNA fragments
To increase the specificity of DNA amplification
To facilitate denaturation of DNA
Help extract DNA from cheek cells
What is the purpose of the Heat Block step at 10 minutes @ 95 degrees Celsius in PCR (Polymerase Chain Reaction) procedure?
To cool down the reaction mixture before the next step
break open cells - release DNA into solution
To activate the DNA polymerase enzyme
To denature the DNA into single strands
What is the function of Taq DNA Polymerase in the PCR (Polymerase Chain Reaction) procedure?
Initiates the PCR reaction by denaturing the DNA template
Adds primers to the DNA template for replication
Facilitates the annealing of primers to the DNA template
Catalyzes building complimentary strand during DNA synthesis (+ has a high heat capacity)
What is the function of PCR Buffer with Mg(^2) in the PCR (Polymerase Chain Reaction) procedure?
Enhances enzyme activity and optimizes stability of primer template
Maintains the optimal pH for enzyme activity
Provides the necessary nucleotides for DNA synthesis
Facilitates the annealing of primers to the DNA template
What is the function of dNTPs in the PCR (Polymerase Chain Reaction) procedure?
Acts as a cofactor for DNA polymerase enzyme
Facilitates the annealing of primers to the DNA template
Provides the necessary nucleotides for DNA synthesis
Provides the necessary energy for DNA amplification
What is the function of Gel loading dye in the PCR Master Mix?
Provides additional charge to DNA molecules for better separation
Stains the DNA to be seen for gel electrophoresis
Adjusts the pH of the gel matrix
Cuts the DNA to make bands
What is the function of PTC Primer Mix in PCR (Polymerase Chain Reaction) procedure?
Increases the specificity of RNA amplification
Facilitates denaturation of DNA strands
Enhances the stability of the DNA template
Contains complementary sequences to the target region for amplification
What is the function of denaturation in PCR (Polymerase Chain Reaction) procedure
Breaks hydrogen bonds to form single-stranded genes
Increases the stability of the DNA template
Facilitates the annealing of primers to the DNA template
Finishes the PCR reaction by bonding with the DNA template
What is the function of annealing in PCR (Polymerase Chain Reaction) procedure?
Increases the temperature to denature the DNA template
Promotes the elongation of the DNA strand
Decreases temperature so primers attach to the template
Facilitates the binding of DNA polymerase to the template strand
What is the function of extension in PCR (Polymerase Chain Reaction) procedure?
Increases the temperature to build complementary strand
Initiates the PCR reaction by denaturing the DNA template
Facilitates the annealing of primers to the DNA template
Increases the stability of the DNA template
What is the purpose of conducting 30 cycles in the PCR (Polymerase Chain Reaction) procedure?
To ensure complete denaturation of the DNA template
To optimize the binding of primers to the DNA template
To amplify the DNA to billions of copies
To stabilize the DNA strands for subsequent analysis
