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WorksheetsSterilization, Disinfection and Antisepsis
Total questions: 22
Worksheet time: 9mins
2. Autoclave method is the most common and reliable sterilization process used in the microbiology laboratory. Which of the following are the recommended heat temperature and time periods for the moist heat sterilization method used in an autoclave?
a. 180 o c for 5 minutes
b. 121o c for 15 minutes
c. 126o c for 3 minutes
d. 160 oc for 45 minutes
4. Which of the following best describes the process of 'Disinfection'?
a. The elimination of all forms of microorganisms and bacterial spores
b. The elimination of all forms of bacterial spores
c. The reduction or elimination of microorganisms and bacterial spores
d. The reduction or elimination of many microorganisms and some bacterial spores
5. Name the sterilization agent that is most frequently used in hospitals and clinical laboratories for the heat-labile liquid substances or antibiotics
a. Dry heat
b. Radiation
c. Filtration
d. Formaldehyde
6. Which of the following methods has been found safe and effective in the elimination of COVID-19?
a. Use of 60-70 % isopropyl alcohol
b. Use of antibacterial ointments
c. Hand wash with Soap and water
d. Intake of antibiotics when symptoms appear
9. Which of the following sterilizing agent is found to have less or no sporicidal activity?
a. Hot air oven
b. Ethyl alcohol
c. Pasteurization
d. Autoclave
10. Name the spore containing the pathogen which shows resistance to heat and is usually not destroyed by using heat at 100 degrees Celsius.
a. E. coli
b. Candida albicans
c. Vibrio cholera
d. Clostridium perfringens
13. Which of the following is the correct definition for the 'pasteurization' process of milk and fermented products?
a. The sterilization method that uses heat at a boiling temperature of 100 degrees Celsius
b. The sterilization method that uses heat at 100 to 120 degrees Celsius
c. The sterilization method that uses moist heat below 100 degrees Celsius
d. The sterilization method that uses moist heat above 100 degrees Celsius
14. Nonionizing radiation and ionizing radiation are sterilization methods mainly used in hospitals. Ultraviolet radiation is one example of nonionizing radiation, name the ionizing radiation?
a. Infrared
b. X-rays and gamma rays
c. Halogens
d. Ethylene oxide
15. Identify the temperature and time period commonly used for the hot air oven while sterilizing glassware in the laboratory
a. 180 degrees Celsius for 30 mins
b. 63 degrees Celsius for 30 mins
c. 121 degrees Celsius for 15 mins
c. 160 degrees Celsius for 45 mins
20. Name the chemical which is an active ingredient of 'bleach', a household decontamination product used to kill bacteria, fungi, and viruses?
a. Sodium chloride
b. Ethylene oxide
c. Sodium hypochlorite
d. Ethyl alcohol
22. When handling items which are soiled with bodily fluids, it’s important to wear gloves. Which of these would not be suitable?
a. Latex
b. Neoprene
c. Polythene
d. plastic
33. Which method of disinfection can be suitable for patient room?
a. Sterilization
b. Fumigation
c. Incineration
d. Autoclaving
37. What is the definition of a ‘single use’ item?
a. An item that can be used by a single patient, multiple times
b. An item that can only be used once before it needs disinfecting
c. An item that can only be used once before being disposed of
d. An item that can only be used for a single day
42. Which of the following is not a physical disinfection method?
a. Heat
b. Sunlight
c. Metals
d. UV
45. What is the chemical or mechanical cleaning process called
a. Sterilization
b. Disinfection
c. Decontamination
d. Sanitization
A surgical nurse is preparing instruments for an upcoming procedure. Which of the following settings should be used in the autoclave for effective sterilization?
a) 121°C at 15 pounds pressure for 10 minutes
b) 121°C at 15 pounds pressure for 15 minutes
c) 160°C at 15 pounds pressure for 1 hour
d) 100°C at atmospheric pressure for 30 minutes
A laboratory technician needs to sterilize glass petri dishes. Which method would be most appropriate?
a) Autoclave at 121°C for 15 minutes
b) Hot air oven at 160°C for 1 hour
c) Bunsen burner flame
d) Ultraviolet radiation
A manufacturer of single-use plastic syringes wants to ensure sterility of the products. Which sterilization method should be employed?
Gamma irradiation
Ultraviolet radiation
Ethylene oxide gas
Filtration through HEPA filters
A microbiologist wants to reduce airborne microbes in the laboratory work cabinet. Which type of radiation would be suitable for surface decontamination?
Gamma radiation
Ultraviolet radiation
X-rays
Infrared radiation
In an operating theatre, it is crucial to maintain a sterile environment. Which type of filter should be used to remove airborne organisms?
Millipore filters
Whatman filter paper
HEPA filters
Cheesecloth
A hospital needs to sterilize heat-sensitive equipment such as heart valves. Which gas sterilant would be most appropriate?
Formaldehyde
Hydrogen peroxide vapor
Ethylene oxide
Chlorine dioxide
An endoscopy unit needs to disinfect heat-sensitive endoscopes between procedures. Which chemical sterilant should be used?
2% Glutaraldehyde solution
70% Ethanol
0.5% Sodium hypochlorite
Quaternary ammonium compounds
