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WorksheetsCheckpoint: CRISPR-Cas9
Total questions: 13
Worksheet time: 10mins
CRISPR-Cas9 is a type of ________ system found in bacteria.
digestive
endocrine
circulatory
immune
CRISPR-Cas9 was discovered in which of the following organisms?
protists
fungi
bacteria
viruses
Scientists have adapted it to turn it into a biotechnology tool for _______ DNA.
building
editing
destroying
synthesizing
The enzyme portion of this complex is called ________.
CRISPR
Replicase
Lysozyme
Cas9
What type of RNA is used as a GPS of sorts, to find or locate specific genes?
mRNA
gRNA
tRNA
rRNA
What is PAM?
a 3 letter sequence for binding on the target DNA
the main gene portion of the DNA
the cut site for Cas9
a 10 letter sequence for binding on the target DNA
Once the DNA and RNA are bound, Cas9 is activated to cut the DNA ____ upstream from the binding sequence.
1 base
3 bases
6 bases
10 bases
What does the acronym CRISPR stand for?
Controlled Reservative Image Spectacle Palindromic Recounts
Clustered Regularly-Interspaced Short Palindromic Repeats
Why do bacteria have CRISPR? Check all that apply.
To be able to remember which viruses they have been infected with
To be able to edit virus DNA
To cut apart virus DNA thereby stopping the virus infection
To be able to edit their own DNA
What is the role of the guide RNA?
It helps the cell repair the DNA
ensures that the Cas9 enzyme cuts at the right point in the genome
Recognizes PAM sequences and opens up the DNA
makes a cut across both strands of the DNA
How does the guide RNA help the cell know where to cut the DNA?
Guide RNA helps to find the PAM
Guide RNA is complementary to the DNA sequence
Guide RNA always binds to the same sequence of DNA
Like restriction sites, they are always palindromes
The following sequence is a known GENE MUTATION that geneticists would like to remove. What would be the guide RNA they need to build into a CRISPR-Cas9 enzyme?
TTA CCG
AAU GGC
AAT GGC
TTA CCG
GCC ATT
We have been modifying DNA sequences for many years - ever since restriction enzymes were discovered in the early 1970's. CRISPR and restriction enzymes are essentially the same because they can both be used to cut DNA and can be used inside of living cells.
TRUE
FALSE - Restriction enzymes do not cut DNA
FALSE - CRISPR does not cut DNA
FALSE - Restriction enzymes cannot be used inside of living cells
