WorksheetsBMD - Primers & Probes
Total questions: 10
Worksheet time: 5mins
Which technique uses primers?
Southern blot
ELISA
PCR
Microarray
A primer is a short _____ sequence that binds to its _____ sequence in the target.
DNA; complementary
DNA; exact
protein; DNA
RNA; exact
What is the sequence of the primer that binds to this target DNA?
5'-GTACTAGGATTAATGCGTTT-3'
5'-GTACTAGGATTAATGCGTTT-3'
3'-GTACTAGGATTAATGCGTTT-5'
5'-AAACGCATTAATCCTAGTAC-3'
3'-AAACGCATTAATCCTAGTAC-5'
Which primer characteristic DOES NOT affect its SPECIFICITY?
Length
GC content
Uniqueness
Melting temperature
What is the melting temperature (Tm) for this primer?
5'-TCA GTG ATG GTG ATG ATG GCG-3'
56'C
60'C
54'C
64'C
What is the annealing temperature for the following primer pair?
Forward primer Tm = 60'C
Reverse primer Tm = 58'C
56'C
58'C
60'C
62'C
Which online tool should be used to check for primer specificity?
PubMed
blast
NCBI
UniProt
A probe is different from a primer because
probe can have modification at its 3' end.
probe must always be longer than a primer.
probe is usually RNA while primer is DNA.
probe binds outside the gene of interest.
Which one of the following is NOT the result of using a short primer?
Low Tm
Low specificity
Low sensitivity
Low stability
A probe can be used in
real-time PCR.
gel electrophoresis.
conventional PCR.
sequencing.
