WorksheetsPMLS prelims week 4 and 5
Total questions: 88
Worksheet time: 47mins
▪ O2 rich blood
▪ CO2 poor blood
- Returns the deoxygenated blood to its
origin (via veins)
Oxygenated blood
Deoxygenated blood
Hemostasis
Vasoconstrict
Vasodilation
▪ CO2 rich blood
▪ O2 poor blood
- Helps in the coagulation process
Oxygenated blood
Deoxygenated blood
Hemostasis
Vasoconstrict
Vasodilation
▪ stoppage of
blood/bleeding
▪ when there’s injury, the
bleeding will stop
because of clotting, then
the clotting will dissolve
through fibrinolysis –
hemostasis is maintained
- Regulates the body temperature
Oxygenated blood
Deoxygenated blood
Hemostasis
Vasoconstrict
Vasodilation
▪ The lumen of the blood
vessel constricts, and
because of the pressure,
rate of blood flow
increases → the body
temperature also
increases
Oxygenated blood
Deoxygenated blood
Hemostasis
Vasoconstrict
Vasodilation
▪ The lumen dilates/widens
which decreases the rate
of blood flow → body
temperature decreases
Oxygenated blood
Deoxygenated blood
Hemostasis
Vasoconstrict
Vasodilation
- Hollow muscular organ
- Four chambers (left atrium, right atrium,
left ventricles, and right ventricles)
- Surrounded by pericardium (thin, fluid-
filled sac)
- About the same size as of a man's
clenched fist
- Less than 1 lb. (250 to 350 grams)
(a)
- Fluid inside pericardium
- Not collected by medtechs, doctors
collect them, but medtechs test them
(a)
- thin, watery membrane in the outer layer
of the heart.
- Function: covers the heart and attached
to the pericardium.
Epicardium
Myocardium
Endocardium
- thick layer of cardiac muscles in the
middle layer of the heart.
- Function: pumps blood to the arteries by
contracting.
Epicardium
Myocardium
Endocardium
- thin layer of epithelial cells in the inner
layer of the heart.
- Function: lines the valves and interior
chambers.
Epicardium
Myocardium
Endocardium
- upper right chamber
- Function: Receives deoxygenated blood
from the body.
Right atrium
Right ventricle
Left Atrium
Left Ventricle
-- lower right chamber
- Function: Receives the blood from the
right atrium and pumps into pulmonary
artery.
Right atrium
Right ventricle
Left Atrium
Left Ventricle
- upper left chamber
- Function: Receives oxygenated blood
from the lungs and pumps it into the left
ventricle.
Right atrium
Right ventricle
Left Atrium
Left Ventricle
- lower left chamber
- Function: Receives blood from the left
atrium and pumps it into the aorta.
Right atrium
Right ventricle
Left Atrium
Left Ventricle
- located between the right atrium and
right ventricle.
- Function: Closes as the right ventricle
contracts preventing blood from flowing
back to the right atrium.
Right AV valve (tricuspid valve)
Left AV valve (bicuspid or mitral valve)
Right semilunar valve (pulmonary/ pulmonic
valve)
Left semilunar valve (aortic valve)
- located between the left atrium and left
ventricle.
- Function: Closes as the left ventricle
contracts preventing blood from flowing
back to the left atrium.
Right AV valve (tricuspid valve)
Left AV valve (bicuspid or mitral valve)
Right semilunar valve (pulmonary/ pulmonic
valve)
Left semilunar valve (aortic valve)
- located at the entrance of the
pulmonary artery.
- Function: Closes when the right ventricle
relaxes preventing blood from flowing
back to the right ventricle.
Right AV valve (tricuspid valve)
Left AV valve (bicuspid or mitral valve)
Right semilunar valve (pulmonary/ pulmonic
valve)
Left semilunar valve (aortic valve)
- located at the entrance of the aorta.
- Function: Closes when the left ventricle
relaxes preventing blood from flowing
back to the left ventricle.
Right AV valve (tricuspid valve)
Left AV valve (bicuspid or mitral valve)
Right semilunar valve (pulmonary/ pulmonic
valve)
Left semilunar valve (aortic valve)
The loop which consists of a system of
blood vessels through which blood is
circulated to the rest of the body
(a)
- Moves the blood between the right
ventricle of the heart to the lungs
- During the process, oxygen is absorbed
and carbon dioxide is released, after which, the oxygenated blood flows back
to the left atrium of the heart
Pulmonary Circulation
Systemic Circulation
- Moves the oxygenated blood and
nutrients from the left ventricle of the
heart to the rest of the body
- The deoxygenated blood with carbon
dioxide and waste flows back to the right
atrium
Pulmonary Circulation
Systemic Circulation
- thick-walled blood vessels that carry
oxygen-rich blood from the heart to the
tissues of the body.
Arteries
Arterioles
Veins
Venules
Capillaries
- a small-diameter blood vessel that
branches out from the arteries and leads
to the capillaries.
Arteries
Arterioles
Veins
Venules
Capillaries
- tubes with thin walls that carry
deoxygenated blood from tissues to the
heart.
Arteries
Arterioles
Veins
Venules
Capillaries
- a very small vein that collects blood from
the capillaries.
Arteries
Arterioles
Veins
Venules
Capillaries
- fine hair-like blood vessels that connect
arterioles and veins
- Where the gas exchange happens
Arteries
Arterioles
Veins
Venules
Capillaries
- Tunica adventitia (outer connective
tissue)
- Tunica media (middle, muscle and
elastic fiber)
- Tunica intima (inner, endothelial cell)
Layers
Lumen
Valves
7.35 - 7.45
ph
pCO2
pO2
HCO2
O2 Sat
35 mmHg to 45 mmHg
ph
pCO2
pO2
HCO2
O2 Sat
75 mmHg to 100 mmHg
ph
pCO2
pO2
HCO2
O2 Sat
22 mEq/L to 26 mEq/L
ph
pCO2
pO2
HCO2
O2 Sat
Greater than 95%
ph
pCO2
pO2
HCO2
O2 Sat
- increase when there is tissue injury
- tested to check the source of injury
Enzymes
Microbial Cultures
Potassium (K)
Lipid Profile
- Bacterial endocarditis
o Overwhelming blood bacteria
o Bacteria can come to the heart
o Can come from sepsis
- Sepsis – bacterial infection in the blood
Enzymes
Microbial Cultures
Potassium (K)
Lipid Profile
Abnormal amount can cause
abnormality in heartbeat – arrythmia
Enzymes
Microbial Cultures
Potassium (K)
Lipid Profile
- Plaque from cholesterol, triglycerides
o Can block the blood vessel
Enzymes
Microbial Cultures
Potassium (K)
Lipid Profile
o Good cholesterol
o Normal value is 40 mg or higher
▪ Even if it is higher, it is fine
o Transfer cholesterol from the
blood vessel to liver for
elimination
High Density Lipoprotein (HDL)
Low Density Lipoprotein (LDL)
o Bad cholesterol
o Absorbs cholesterol and deposit
to the blood vessel
High Density Lipoprotein (HDL)
Low Density Lipoprotein (LDL)
- Clot can block blood flow that is why it
undergoes fibrinolysis
- Fibrinolysis → fibrin degradation products
→ ____dissolves → checks if it can dissolve FDP
Prothrombin Time
D-Dimer and FDP
- to form a stable clot to stop bleeding
- abnormal if you lack factors I, II, V, VII, X
- Factor VIII – hemophiliac A
- Factor IX – hemophiliac B
- Factor XI – hemophiliac C
Prothrombin Time
D-Dimer and FDP
- a clear, straw-colored liquid portion of
the blood which is 90% water
(a)
bright red
Arterial Blood
Venous blood
dark red
Arterial Blood
Venous blood
Universal donor as it has no antigen
Blood Type O
Blood Type AB
- Universal recipient as it has no antibodies
Blood Type O
Blood Type AB
- Blood bag for blood transfusion
- Comes from whole blood
- Has no plasma since it will contain
antibodies
Packed RBC
Blood component preparation
Blood typing
Anti-Sera
- Components of blood are divided for
specific needs
Packed RBC
Blood component preparation
Blood typing
Anti-Sera
- Forward slide method
- What clotted, that is the blood type
Packed RBC
Blood component preparation
Blood typing
Anti-Sera
- Used for blood typing procedure
- Acts as a plasma
- Has three types: Anti A, Anti B, Anti D
- Anti A target is A, Anti B target is B, Anti D
target is D
Packed RBC
Blood component preparation
Blood typing
Anti-Sera
- Phlebotomy Section/Area
- Where phlebotomy procedures are
performed
- Typically contains
o Table for supplies
o Special chair
o Bed or reclining chair
o *Bed or padded table
Blood Drawing Station
Phlebotomy Chairs
Equipment Carriers
Gloves and Glove Liners
Antiseptic
- comfortable
- have adjustable armrests
Blood Drawing Station
Phlebotomy Chairs
Equipment Carriers
Gloves and Glove Liners
Antiseptic
- Make blood collection equipment
portable
- Handheld Carriers
o Easily carried
o Contain enough equipment for
numerous blood draw
o “stat” or emergency situations
- Phlebotomy Carts
o Gliding carts made up of steel or
synthetic material
o Shelves carrying adequate
supplies for many patients
o Not in the room, hallway only,
nosocomial infection
Blood Drawing Station
Phlebotomy Chairs
Equipment Carriers
Gloves and Glove Liners
Antiseptic
- One for each patient
- Non sterile, disposable latex, nitrile,
neoprene, polyethylene and vinyl
- Special gloves
- Barrier hand creams
- KEYPOINT: Decontamination of hands
after glove removal is ESSENTIAL.
Blood Drawing Station
Phlebotomy Chairs
Equipment Carriers
Gloves and Glove Liners
Antiseptic
- Substances that are used to prevent
sepsis
Blood Drawing Station
Phlebotomy Chairs
Equipment Carriers
Gloves and Glove Liners
Antiseptic
- Chemical substances used to remove or
kill microorganisms on surface and
instruments
Disinfectants
Hand Sanitizers
Gauze Pads
Bandages
- - Detergent containing wipes for visibly
soiled hand and there’s no hand
washing facilities
Disinfectants
Hand Sanitizers
Gauze Pads
Bandages
Hold pressure over the site following
blood collection procedure
- USE OF COTTON BALLS TO HOLD
PRESSURE IS NOT RECOMMENDED
Disinfectants
Hand Sanitizers
Gauze Pads
Bandages
- Used to cover a blood collection site
after the bleeding has stopped
- Paper, cloth, knitted tape
- Caution: Adhesive bandages should not
be used on babies younger than 2 years
of age because of aspiration and
suffocation
Disinfectants
Hand Sanitizers
Gauze Pads
Bandages
- Where you place used needles, lancets,
and other sharp objects
- With BIOHAZARD SYMBOL
Needles and Sharps Disposal Containers
Biohazard Bags
Gauze Pads
Bandages
- Leakproof plastic bags
- Transporting blood and other specimens
from collection site to the laboratory
- BIOHAZARD label and outside pocket
Needles and Sharps Disposal Containers
Biohazard Bags
Gauze Pads
Bandages
- Can be performed by means of 3
different methods:
o Syringe method
o Butterfly infusion method
o Vacutainer/ETS method
(a)
- Portable transillumination devices
o Transillumination - Inspecting
organ by passing light through its
wall
- Principle: Hemoglobin in the blood within
the veins absorbs the light, causing the
vein to stand out as dark lines
Vein Locating Device
Tourniquet
- Purpose:
o Constrict the flow of blood
o Makes the vein more prominent
- Flat strip, non-latex made material
- Can be reused
Vein Locating Device
Tourniquet
commonly used
20-21g
21 and 22
23 g
21-23 and 25
22 used for blood collection
20-21g
21 and 22
23 g
21-23 and 25
used for children
20-21g
21 and 22
23 g
21-23 and 25
used for butterfly
infusion/needle
20-21g
21 and 22
23 g
21-23 and 25
PPD skin test
27 gauge
25 gauge
23 gauge
22 and 21 gauge
18and 16 gauge
Butterfly or Syringe Collection
27 gauge
25 gauge
23 gauge
22 and 21 gauge
18and 16 gauge
Butterfly or Syringe CollectionSyringe or Evacuated system
collection
27 gauge
25 gauge
23 gauge
22 and 21 gauge
18and 16 gauge
Ivs or Blood donation
27 gauge
25 gauge
23 gauge
22 and 21 gauge
18and 16 gauge
- Interrupt the process of coagulation
- EDTA, Citrate, Heparin, Oxalates
Anticoagulants
Anti-glycolytic
- Prevents Glycolysis / Sodium Fluoride
Anticoagulants
Anti-glycolytic
Action: Chelates Calcium
Ethylenediaminetetraacetic acid (EDTA)
Citrate
Heparin
Oxalates
Combines calcium in a non-
ionized form
Ethylenediaminetetraacetic acid (EDTA)
Citrate
Heparin
Oxalates
Acts as antithrombin and
antithromboplastin
Ethylenediaminetetraacetic acid (EDTA)
Citrate
Heparin
Oxalates
Combines with Calcium to form
an insoluble salt.
Ethylenediaminetetraacetic acid (EDTA)
Citrate
Heparin
Oxalates
Forming weakly dissociated
calcium components
Fluoride
Sodium Polyanethol Sulfonate
Serum Separator Tube
Plasma Separator Tube
Gold Top
- Design to help isolate
Fluoride
Sodium Polyanethol Sulfonate
Serum Separator Tube
Plasma Separator Tube
o Anti-coagulant
o Anti-complementary
o Anti-phagocytic
o Neutralizes aminoglycoside
(antibiotic)
Fluoride
Sodium Polyanethol Sulfonate
Serum Separator Tube
Plasma Separator Tube
White Top
- In order of draw: After SST or before
Heparinized tube
Fluoride
Sodium Polyanethol Sulfonate
Serum Separator Tube
Plasma Separator Tube
order of draw by color
(a)
Sterile blood culture tubes-
yellow
lightblue
red
green
lavander
Coagulation tubes-
yellow
lightblue
red
green
lavander
Serum tube/ Plain tube-
yellow
lightblue
red
green
lavander
Heparin tube-
yellow
lightblue
red
green
lavander
EDTA tube-
yellow
lightblue
red
green
lavander
Anti-glycolytic tube/ Fluoride-
yellow
lightblue
red
green
gray
