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Basics of Chromatography

Total questions: 15

Worksheet time: 9mins

Name
Class
Date
1.

Chromatography is a physical method that is used to separate and analyze.....

a)

simple mixtures

b)

complex mixtures

c)

viscous mixtures

d)

metals

2.

In which type of chromatography, the stationary phase held in a narrow tube and the mobile phase is forced through it under pressure?

a)

column chromatography

b)

planar chromatography

c)

liquid chromatography

3.

In chromatography, the stationary phase can be _____ supported on a solid.

a)

liquid or gas

b)

solid or liquid

c)

solid only

d)

liquid only

4.

In chromatography, which of the following can be mobile phase be made of?

a)

solid or liquid

b)

liquid or gas

c)

gas only

d)

liquid only

5.

Chromatography cannot be used to purify volatile substances.

a)

True

b)

False

6.

The retention factor is used to...

a)

zero the chromatography scale

b)

retain the solvent

c)

identify unknown compounds in a sample

d)

as a starting tool for chromatography

7.

The Rf value for the blue component is..

a)

0.3

b)

0.7

c)

10

d)

3

8.

Why must the base line in TLC be drawn in pencil and not pen?

a)

pencil is good at keeping substances in place

b)

pencil is soluble in water

c)

pencil is insoluble in water

d)

pencil is not colored

9.

The substance in the mixture that has the greatest affinity for the mobile phase will travel...

a)

fastest

b)

slowest

10.

A student extracts the color from some green smarties and uses paper chromatography to separate components colors. She finds blue has a Rf=0.38, and yellow Rf=0.75. This suggests...

a)

yellow is more strongly adsorbed onto the stationary phase and is less soluble in mobile phase.

b)

the yellow is more strongly adsorbed onto the stationary phase and is more soluble in the mobile phase.

c)

the blue is more strongly adsorbed onto the stationary phase and is less soluble in the mobile phase.

d)

the blue is more strongly adsorbed onto the stationary phase and is less soluble in the mobile phase.

11.

The Rf value for the red component is...

a)

0.3

b)

10

c)

0.7

d)

7

12.

Which plant species has plant pigments similar to those in A?

a)

B

b)

C

c)

D

13.

If a substance is not soluble in the solvent, what happens to the spot placed on the start line?

a)

Dot moves to the top of the paper

b)

Dot moves to the left or right

c)

Dot does not move at all

d)

Dot moves down to the bottom of the paper

14.

Whose blood stain was found at the crime scene?

a)

Bob

b)

Lisa

c)

John

d)

Sue

15.

The diagram shows a chromatogram obtained when a sample X was analyzed together with four other known dyes P, Q,R and S. Dye Q was known to cause cancer. Which of the following dye(s) is/are safe for use?

a)

P only

b)

P and S

c)

S only

d)

P and X