WorksheetsPA2 FINALS
Total questions: 50
Worksheet time: 3hrs 30mins
Name
Class
Date
1.
process of extracting one material from another material by washing with a solvent (eluant)
transport of solute/sample (eluent) by continuous addition of mobile phase
a)
Polarimetry
b)
Potentiometry
c)
Spectrometry
d)
Chromatography
2.
Column chromatography and Thin layer chromatography are examples of..
a)
Adsorption Chromatography
b)
Gas-Liquid chromatography
3.
type of partition chromatography in which the SP is a layer of water adsorbed on a sheet of paper
a)
Paper chromatography
b)
Gas chromatography
c)
High performance liquid chromatography
4.
Method of choice for inorganic ions and attempts of reversed phase method are unsuccessful
a)
Ion exchange
b)
Partition Chromatography
c)
Adsorption Chromatography
d)
Size exclusion
5.
Used in separation of
solutes with different molecular size,
large molecules, macromolecular complexes such as proteins and industrial polymers
a)
Size exclusion
b)
Ion exchange
c)
Partition Chromatography
d)
Adsorption Chromatography
6.
Size exclusion chromatography used in separation of polar species
a)
Gel Filtration Chromatography
b)
Gel Permeation Chromatography
7.
Size exclusion chromatography used in separation of non-polar species
a)
Gel Filtration Chromatography
b)
Gel Permeation Chromatography
8.
The migration of electrically charged molecules suspended and dissolved in an electrolyte under the influence of an external electrical field (electric current)
a)
Electrophoresis
b)
Ion exchange
c)
Partition Chromatography
d)
Adsorption Chromatography
9.
The migration of electrically charged molecules suspended and dissolved in an electrolyte under the influence of an external electrical field (electric current)
a)
Electrophoresis
b)
Ion exchange
c)
Partition Chromatography
d)
Adsorption Chromatography
10.
widely used especially for the separation of complex mixtures of biological substances such as proteins, nucleic acids and polysaccharides
a)
Electrophoresis
b)
Ion exchange
c)
Partition Chromatography
d)
Adsorption Chromatography
11.
Differences of partition coefficients of substances between two immiscible liquids
a)
Paper chromatography
b)
Column chromatography
c)
Thin layer chromatography (TLC)
12.
Allow the mobile phase to flow downward on the paperclip
a)
Descending Chromatography
b)
Ascending Chromatography
c)
Radial Chromatography
13.
mobile phase is allowed to rise upward on the paper by capillary attraction
a)
Descending Chromatography
b)
Ascending Chromatography
c)
Radial Chromatography
14.
mobile phase moves out in concentric circles from the center of circular piece of paper
a)
Descending Chromatography
b)
Ascending Chromatography
c)
Radial Chromatography
15.
Stationary phase is held within or on the surface of a narrow tube, Mobile phase is forced through the tube, where compound separation occurs
a)
Paper chromatography
b)
Column chromatography
c)
Thin layer chromatography (TLC)
16.
liquid-solid chromatography in which the stationary phase is a thin layer on the surface of an appropriate plate
a)
Paper chromatography
b)
Column chromatography
c)
Thin layer chromatography (TLC)
17.
TLC Detection Methods: Dipping the TLC plate in sulfuric acid and then heating it can char organic compounds, making them visible as black spots.
a)
Charring
b)
Iodine vapor
c)
Examination of plate under UV radiation
18.
TLC Detection Methods: TLC plates are exposed to iodine vapor, which temporarily stains many organic compounds brown or yellow.
a)
Charring
b)
Iodine vapor
c)
Examination of plate under UV radiation
19.
TLC Detection Methods: Used for compounds that fluoresce under UV light, making them visible as bright spots against a dark background.
a)
Charring
b)
Iodine vapor
c)
Examination of plate under UV radiation
20.
Involves a sample being vaporized and injected onto the head of the chromatographic column
a)
Adsorption Chromatography
b)
Gas-Liquid chromatography
21.
Gas chromatography is limited to volatile samples
a)
TRUE
b)
FALSE
22.
Gas chromatography is suitable for thermally labile samples
a)
TRUE
b)
FALSE
23.
Used in characterization of volatile oils
a)
Paper chromatography
b)
Gas chromatography
c)
High performance liquid chromatography
24.
In Gas Chromatography, temperature of the sample port is usually about 50C higher than the boiling point of the most volatile component of the sample
a)
TRUE
b)
FALSE
25.
Most advance chromatographic method
a)
Paper chromatography
b)
Gas chromatography
c)
High performance liquid chromatography
26.
Measures of the fraction of its total elution time that any compound spends in the mobile phase
a)
Retardation Factor
b)
Retardation Fraction
c)
Elution Fraction
d)
Retention Factor
27.
the time that elapses from the start of the chromatogram to the elution maximum of the solute
a)
Retention time
b)
Retention factor
c)
Retention fraction
d)
Retardation time
28.
It is equal to the volume of the MP required to elute a compound from the system
a)
Retardation Volume
b)
Retention Volume
c)
Elution Volume
d)
Retardation Factor
29.
Component of HPLC where solute mixture is introduced
a)
Injection Valve
b)
Column / Analytical Column
c)
Detector
d)
Integrator
30.
Component of HPLC that measures concentration of sample injected into the column
a)
Injection Valve
b)
Column / Analytical Column
c)
Detector
d)
Integrator
31.
The measurement of optical rotation or optical or optical activity of a substance
a)
Polarimetry
b)
Potentiometry
c)
Chromatograpy
d)
Spectrometry
32.
a chiral center is a carbon that has 3 different group attached to it
a)
TRUE
b)
FALSE
33.
Clockwise rotation (+ isomer)
a)
Dextrorotatory
b)
Levorotatory
34.
Counterclockwise rotation (-isomer)
a)
Dextrorotatory
b)
Levorotatory
35.
Substance that rotates plane polarized light to the left or to the right are optically active
a)
TRUE
b)
FALSE
36.
Converts light from the source into polarized light.
a)
Light Source
b)
Polarizer
c)
Sample Tube
d)
Analyzer
e)
Detector
37.
Contains the optically active sample solution.
a)
Light Source
b)
Polarizer
c)
Sample Tube
d)
Analyzer
e)
Detector
38.
Rotated to determine the angle at which light passes through, allowing measurement of the angle of rotation
a)
Light Source
b)
Polarizer
c)
Sample Tube
d)
Analyzer
e)
Detector
39.
Detects the intensity of the transmitted light and helps in quantifying the angle of rotation
a)
Light Source
b)
Polarizer
c)
Sample Tube
d)
Analyzer
e)
Detector
40.
Polarimetry is only applicable to optically active substances
a)
TRUE
b)
FALSE
41.
Branch of electrochemistry which deals with the study and measurement of electrode potential
a)
Polarimetry
b)
Potentiometry
c)
Spectrometry
d)
Chromatography
42.
Provides a stable and known potential.
a)
Reference Electrode
b)
Indicator Electrode
c)
Potentiometer
43.
Its potential changes with the concentration of the analyte ion
a)
Reference Electrode
b)
Indicator Electrode
c)
Potentiometer
44.
A device that measures the potential difference between the reference and indicator electrodes
a)
Reference Electrode
b)
Indicator Electrode
c)
Potentiometer
45.
Measures the potential of the indicator electrode directly related to the analyte concentration.
a)
Direct potentiometry
b)
Potentiometric Titration
46.
Commonly used for pH measurements and specific ion concentration measurements
a)
Direct potentiometry
b)
Potentiometric Titration
47.
The potential is measured as a titrant is added to the analyte solution
a)
Direct potentiometry
b)
Potentiometric Titration
48.
The endpoint of the titration is indicated by a sharp change in potential
a)
Direct potentiometry
b)
Potentiometric Titration
49.
The most common application of potentiometry, using a glass electrode as the indicator electrode.
a)
pH Measurement
b)
Ion-Selective Electrode (ISE) Measurements
c)
Redox Potentiometry
50.
Used to measure specific ions such as Na+, K+, Ca2+, Cl−, and others.
a)
pH Measurement
b)
Ion-Selective Electrode (ISE) Measurements
c)
Redox Potentiometry
100 %
