WorksheetsMethods in Gene Cloning
Total questions: 10
Worksheet time: 5mins
Which of the followings is NOT a step that involved in gene cloning?
Screen the bacterial cell that contain recombinant DNA.
Insert DNA fragments into open plasmid and join them using DNA ligase.
Cut the plasmid and target DNA with modifying enzyme.
Isolate the plasmid from bacterial cell and target DNA from donor cell.
What is the purpose of screening?
to identify host cell containing plasmid
to identify host cell that able to produce β-galactosidase
to identify host cell containing the recombinant DNA
to identify host cell with DNA
Which of these pairs is INCORRECTLY matched?
DNA ligase - mapping human chromosome
Target DNA - gene of interest
Taq polymerase - PCR
Plasmid as vector - isolated from bacteria
Marker gene that encodes for resistant to ampicillin is ampR. What will happen if the plasmid lack of this gene.
Transformed bacteria can be separated from non transformed bacteria.
Non-transformed bacteria cannot be separated from transformed bacteria.
Transformed bacteria are able to survive in antibiotic ampicillin.
Transformed bacteria are able to form white colony.
What is the purpose of amplification?
To screen for recombinant plasmid
To join the recombinant DNA with the host cell
To produce multiple copies of the desired gene
To prevent the bacteria from reproducing
During the amplification of target gene, an antibiotic medium used to
select for plasmids containing particular DNA fragments
select against plasmids containing human DNA fragments
select for bacteria lacking plasmids
select for bacteria containing plasmids
Which of the following is the CORRECT sequence in gene cloning?
Screening → Isolation → Cut → Insertion → Transformation and amplification
Isolation → Cut → Insertion → Screening → Transformation and amplification
Screening → Transformation and amplification → Cut → Insertion → Isolation
Isolation → Cut → Insertion → Transformation and amplification → Screening
What is transformation in recombinant DNA technology?
Synthesis of mRNA from a DNA molecule
Insertion of mRNA into host cell
Insertion of recombinant DNA into host cell
Synthesis of DNA molecule using mRNA template
Which genetic marker will be altered if a plasmid accepted a gene of interest during cloning process?
lacY gene
lacA gene
lacZ gene
Ampicilin resistance gene
In order to insert a human gene into plasmid, both must
code for the same gene product.
be cut by the same restriction enzyme.
originate from the same type of cell.
have identical sequences.
