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WorksheetsREVISION WEEK 8
Total questions: 14
Worksheet time: 18mins
What is recombinant DNA technology?
Recombinant DNA technology is a method used to join together DNA from different organisms to produce desired traits.
The enzyme that joins foreign DNA fragments with the cloning vector to form recombinant DNA is called (a) .
Which of the following is NOT a tool used in recombinant DNA technology?
Target DNA
Cloning vector
DNA polymerase
Host cell
Name one example of a restriction enzyme used in recombinant DNA technology.
Which type of vector can carry the largest amount of foreign DNA?
Plasmid
Bacteriophage
Cosmid
Yeast artificial chromosome (YAC)
Draw a plasmid used in recombinant DNA technology and label the following parts:
• Origin of replication (ori)
• Selectable marker (antibiotic resistance gene)
• lac Z
• Multiple cloning site (MCS)
• Gene of interest (insulin gene)
Illustrate the process of cleaving a plasmid using the EcoRI restriction enzyme
The method used to determine if a bacterial colony contains the recombinant plasmid is called (a) .
List one application of recombinant DNA technology.
What is the primary purpose of gene cloning in bacteria?
To create genetically modified animals
To produce proteins like insulin
To induce mutations in plants
To create new species
What is the role of restriction enzymes in recombinant DNA technology?
They replicate DNA
They cut DNA at restriction site
They join DNA fragments together
They amplify DNA using PCR
What is the first step in the process of gene cloning?
Screening for the recombinant plasmid
Cleaving the plasmid and target DNA with restriction enzymes
Isolation of the gene of interest
Amplifying the cloned gene with PCR
After the gene of interest is isolated and cleaved, it is inserted into the plasmid at the ___________.
Replication site
Transcription site
Restriction site
Translation site
What happens after the recombinant plasmid is introduced into the host cell?
The plasmid is discarded after use
The host cell replicates and expresses the recombinant DNA
The plasmid is destroyed by the cell
The host cell deactivates the recombinant DNA
