WorksheetsBiotechnology HW 8.2
Total questions: 15
Worksheet time: 8mins
What is the general term for the uptake and expression of foreign DNA by a cell?
Transduction
Transformation
Transcription
Translation
Which company revolutionized medicine by isolating the human insulin gene and inserting it into bacteria cells?
Pfizer
Genentech, Inc.
Merck & Co.
Johnson & Johnson
What is the process called when viruses are used to transform cells?
Transfection
Transduction
Transformation
Translation
What type of cells are commonly used as host cells for mammalian transformation?
Bacterial cells
Yeast cells
Chinese hamster ovary cells
Plant cells
What is the primary use of BACs and YACs in biotechnology?
To transform cells with small pieces of DNA
To host pieces of foreign DNA up to 500 kb in size
To create proteins from DNA sequences
To replicate RNA strands
What is a plasmid?
A large, linear DNA molecule
A small, circular, double-stranded DNA molecule
A protein that binds to DNA
A type of RNA molecule
What is the role of restriction enzymes in DNA manipulation?
To replicate DNA strands
To produce sticky ends by cutting DNA at specific sequences
To synthesize RNA from DNA
To bind proteins to DNA
What is the purpose of DNA ligase in the production of recombinant DNA?
To cut DNA strands
To join matching sticky ends together
To replicate DNA molecules
To synthesize proteins
What is the role of restriction enzymes in bacteria?
To enhance DNA replication
To defend against invading viral DNA
To increase mutation rates
To facilitate protein synthesis
What is a palindrome in the context of restriction enzyme sequences?
A sequence that reads the same forward and backward
A sequence that forms a loop
A sequence that is unique to each organism
A sequence that is resistant to mutation
What is the significance of restriction fragment length polymorphism (RFLP) analysis?
It helps in DNA replication
It reveals differences in DNA sequences
It increases the rate of transcription
It assists in protein folding
What is the unique pattern that results from the DNA analysis of an individual called?
DNA sequence
DNA fingerprint
DNA mutation
DNA replication
What does PCR in DNA fingerprinting target?
Entire genome
Specific sections of genetic information
Only mutations
Non-coding regions
What is the name of the process in which mammalian cells receive and express rDNA?
Transformation
Transduction
Conjugation
Transcription
Which two types of enzymes are needed to produce a rDNA molecule?
Restriction enzymes and ligase
Polymerase and helicase
Ligase and helicase
Polymerase and restriction enzymes
