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WorksheetsBiotechnology HW 8.3
Total questions: 11
Worksheet time: 6mins
What is the role of heat shock in the transformation process?
To enlarge pores and allow plasmids to enter cells
To purify proteins from broth cultures
To discard the cell pellet
To grow transformed cells on selection media
What is the definition of "competent" in the context of cell transformation?
The ability of cells to take up DNA
The process of protein purification
The growth of cells on selection media
The discarding of broth
What is the purpose of using selection media in the transformation process?
To show that cells are producing new proteins
To make cell membranes porous
To discard the broth
To purify proteins
What is the purpose of adding CaCl₂ to cells during the transformation process?
To increase transformation efficiency
To decrease transformation efficiency
To induce cell death
To prevent DNA replication
What is the role of the beta-galactosidase gene in transformed cells?
It produces an enzyme that converts X-gal into a blue product
It provides resistance to antibiotics
It allows cells to grow in the presence of ampicillin
It prevents the growth of non-transformed cells
What is the purpose of a recovery period after cell transformation?
To allow cells to repair their membranes and express new genes
To immediately test for antibiotic resistance
To induce cell death in non-transformed cells
To increase the temperature of the cells
What is a common method to determine if cells have been successfully transformed?
Using a selection medium that kills non-transformed cells
Measuring the size of the cells
Counting the number of cells
Observing the color change in the medium
Which gene is commonly used for selection due to its fluorescent properties?
β-gal gene
Plasmodium sp gene
Green fluorescent protein (GFP) gene
Anopheles sp gene
What is the purpose of the scale-up process in biotechnology?
To decrease the size of the production tanks
To introduce a gene into mosquitoes
To produce large volumes of transformed cells
To disrupt the β-gal enzyme
List two techniques used to increase transformation efficiency.
Heat shock and electroporation
Centrifugation and filtration
Distillation and evaporation
Chromatography and spectrometry
What is GFP and why is it important in genetic engineering work?
A fluorescent protein used as a reporter molecule
A type of enzyme used for digestion
A carbohydrate used for energy storage
A lipid used for membrane structure
