WorksheetsQuiz on Cryopreservation of Cultured Animal Cells
Total questions: 15
Worksheet time: 8mins
What is the primary purpose of cryopreservation?
To promote cell death
To increase cell differentiation
To preserve living cells and tissues
To enhance cell growth
Which factor is crucial for maximizing cell viability upon thawing?
Increasing cell concentration
Rapid cooling
Minimizing intracellular ice crystal formation
Using non-penetrating cryoprotectants
What is the best cryoprotectant mentioned for freezing cells?
Trehalose
Glycerol
PEG
DMSO
What is a significant risk associated with rapid cooling of cells?
Improved membrane function
Enhanced cryoprotectant efficacy
Cold shock damage
Increased cell growth
What is the recommended cooling rate for controlled freezing?
1°C/min
5°C/min
10°C/min
0.5°C/min
Which type of cryoprotectant does not cross the cell membrane?
DMSO
Trehalose
Polyvinylpyrrolidone
Glycerol
What is the main concern when transporting frozen ampoules?
Oxygen exposure
Light exposure
Cell growth
Temperature fluctuations
What should be done to minimize genetic drift in cell cultures?
Increase cell concentration
Replace stocks regularly
Use more cryoprotectants
Decrease freezing time
What is the purpose of using a hydrophilic cryoprotectant?
To increase intracellular ice formation
To enhance cell differentiation
To sequester water
To promote cell growth
Which of the following is a benefit of cryopreservation?
Increased cell differentiation
Generation of safety stocks
Reduction of cell viability
Enhanced cell metabolism
What is the main function of cryoprotectants during freezing?
To promote cell growth
To prevent excessive dehydration
To increase intracellular ice formation
To enhance cell death
What is a common method for thawing cells post-cryopreservation?
At room temperature
In a water bath at 37°C
In a freezer
Under direct sunlight
What is the role of liquid nitrogen in cell preservation?
To promote cell growth
To increase temperature
To enhance cell differentiation
To provide long-term storage
Which of the following is a characteristic of a cell bank?
Allows unrestricted access
Provides secure storage of validated cell lines
Promotes genetic drift
Increases contamination risk
What is the primary concern with residual cellular DNA in cell culture products?
It can lead to tumorigenesis
It improves cell viability
It reduces contamination
It enhances cell growth
