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WorksheetsRecombinant DNA Review 2024-25
Total questions: 23
Worksheet time: 30mins
Which is true about plasmids?
Plasmids can be cut at specific sequences called restriction sites.
Plasmids contain a promoter sequence that defines where transcription begins.
Plasmids can contain an antibiotic resistance gene.
All of the statements are true about plasmids.
Which enzyme helps fuse the foreign into the new DNA?
DNA Polymerase
DNA ligase
The cell that able to receive recombinant DNA for cloning purpose known as
vector
target DNA
plasmid
host cell
To produce desired protein _____________ should be taken from any source like human, animal and plant.
recombinant DNA
Target DNA
plasmid
ligase
During insertion step,
The recombinant plasmid is inserted into a host cell
The gene of interest is inserted into a plasmid
The plasmid is inserted into the gene of interest
In order to insert a human gene into plasmid, both must
code for the same gene product.
be cut by the same restriction enzyme.
originate from the same type of cell.
have identical sequences.
An organism that contains DNA from another organism is called:
transatlantic
recombinant
binary
selective species
What is this
plasmid
recombinant bacterium
recombinant DNA
sticky ends
The process to the right is best used for which of the following?
Cloning humans
Creating antibiotics
Creating human insulin
Causing cells to divide
Which technique would most likely be used by forensic scientist?
Gene cloning
Gene therapy
DNA fingerprinting
Karyotyping
What is golden rice and why is it an example of a GMO?
Golden rice is rice that has recombinant DNA to make it orange. Orange color is preferable to villagers, so it encourages them to eat more.
Golden rice is a type of rice that has recombinant DNA which makes the rice create the metal, "gold." The gold can be used as currency in local villages to improve poverty conditions.
Golden rice is rice that has recombinant DNA so the rice has beta carotene, an important nutrient. Villagers use this rice to get a nutritious diet to improve their eyesight.
Golden rice is a type of rice that only grows in Asia and Africa. It is golden in color, but does not express any genes from other species
What is the structure of DNA?
A circular strand of nucleotides twisted together.
Two strands of nucleotides arranged in a double helix.
A double helix of nitrogenous bases twisted together.
A single strand of nucleotides arranged in a helix.
What is a GMO?
A genetically modified organism that has half of its DNA from one parent and half from the other.
A genetically modified organism that is made of original DNA of the species, plus new DNA the scientist inserted.
A genetically modified organism that has its original DNA cut into pieces, and organized into a new order.
A genetically modified organ where scientists create new organs by pasting different people's DNA together.
How is recombinant DNA made?
Scientists cut the original DNA and the DNA they would like to insert with ligase and then paste them together with restriction enzymes.
Scientists first paste the original DNA and the new DNA together with DNA ligase, then cut them into pieces with restriction enzymes.
Scientists cut up one piece of DNA and then paste the pieces together in a new order with DNA ligase.
Scientists cut the original DNA and DNA they would like to insert with restriction enzymes, then paste them together with DNA ligase
Recombinant DNA technology
is possible only between closely related species
uses bacteria to make copies of the desired product
uses bacteria to make copies of the desired product and splices DNAs together.
uses bacteria to make copies of the desired product, splices DNAs together, and is possible only between closely related species
In order for DNA molecules to undergo recombination they
they must be from the same species
their strands must separate as in replication
must be cut and spliced at specific nucleotide sequences
undergo lysis
Which of the following enzymes are NOT used in the production of a recombinant DNA?
DNA polymerase
DNA ligase
Restriction enzymes
Restriction endonuclease
The first human hormone produced successfully by using DNA recombinant technology is
human growth hormone
insulin
adenosine deaminase
thyroxin
The following are the uses of
recombinant DNA EXCEPT
Produce sheep clone
Produce agriculturally important plants and animals
Produce plants that have bacterial genes to destroy pathogenic insects
Produce microbes that secrete enzymes to overcome the problems of oil spoilage in the sea
Restriction enzymes are used to ___ DNA.
glue together
cut
reconnect
exchange
Which of the following is a common vector used in genetic engineering?
Yeast
Virus
Bacterium
Plasmid
What is the role of a promoter in a plasmid?
To bind restriction enzymes
To initiate DNA replication
To terminate transcription
To initiate transcription
Which of the following is NOT a step in the process of creating recombinant DNA?
Cutting DNA with restriction enzymes
Sequencing the entire genome
Isolation of DNA
Amplifying DNA using PCR
