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WorksheetsCULTURING BACTERIA
Total questions: 15
Worksheet time: 3hrs 30mins
Culture media: nutrient material prepared for bacterial growth in the laboratory. Which one of the following statements is true regarding the differences and uses of blood agar plate and chocolate agar?
Blood agar plate is used for the growth of fastidious organisms, while chocolate agar is not.
Chocolate agar contains lysed red blood cells, while blood agar contains intact red blood cells.
Blood agar plate must be incubated in a dark environment, while chocolate agar does not have this requirement.
Both blood agar and chocolate agar are identical in their nutrient composition.
Liquid media is called
agar
broth
Flaming the neck of the test tube after uncapping the tube and before recapping is essential during culture transfer.
True
False
What is the inoculation loop used for?
Nutrients for bacteria
Sterilising equipment
Collecting/spreading bacteria
Stopping contamination
What is the bunsen burner used for?
Nutrients for bacteria
Sterilising equipment
Collecting/spreading bacteria
Stopping contamination
How can we avoid the agar plate being cross contaminated with unwanted microorganisms when it is open? Pick all that apply?
Work near a bunsen
Wipe the surface of the agar with antiseptic
Minimise the time the lid is open and close it as soon as you are finished inoculation
Pass the agar through the bunsen flame
How should agar plates be incubated
Right side up
Upside down
Sideways
All of the above
The chemical growth requirements essential for bacterial growth are ...?
Water,carbon,Nitrogen & sulphur
Water
Water,carbon,oxygen,phosphorus,Nitrogen,sulphur and other trace elements
Environmental waste
When we refer to 'growth' with bacteria, we are talking about the population and not the size of the bacteria.
False
True
One bacteria found in fresh shell eggs is
Salmonella
Clostridium botulinum
Clostridium perfringens
Staphylococcus aureus
Which type of culture media stimulate the growth of bacteria and inhibit its competitors?
Basal Media
Enriched Media
Selective Media
Indicator Media
Which of the following is an organic component of Enriched media?
amino acids
glucose
water
blood
What is the bet pH for most bacterial growth?
2
4
7
11
Regardless of the media type, the best temperature for most bacteria is
10 -15o C
25 - 40oC
60oC
100oC
What is the difference between nutrient agar and nutrient broth.
one is liquid and one is solid
one is sterile and one is not sterile
one is acidic and one is basic
one has extra sugar and one does not
