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WorksheetsHML2153 - Lesson 2.1 - Microscopy Basics
Total questions: 25
Worksheet time: 2hrs 5mins
Who is credited with coining the term "microscope"?
Hans and Zaccharias
Galileo
Antonie van Leeuwenhoek
Christopher Scheiner
What type of lens is used in a simple microscope?
Concave lens
Plano lens
Convex lens
Cylindrical lens
What does the term "Neofluar" signify in microscopy?
High chromatic aberration
UV light transmission
Oil immersion compatibility
Plano field view
What property of light is most relevant for resolution?
Amplitude
Wavelength
Speed
Phase
What is the function of a microscope condenser?
Magnify the specimen
Focus light onto the specimen
Adjust the tube length
Correct chromatic aberration
What does "numerical aperture" measure?
A) Magnification power
B) Light-gathering ability of the lens
C) Angle of the objective
D) Resolution limit
Which lens type is highly corrected for chromatic aberration?
Plan
Apochromatic
Dry lens
Immersion
What is "empty magnification"?
High magnification without increased detail
Magnification beyond tube length
Magnification without eyepieces
Low numerical aperture magnification
What is the primary role of eyepieces in a microscope?
Focusing the objective lens
Magnifying the image from the objective
Correcting spherical aberration
Reducing light scatter
Which type of aberration results in specimen appearing distorted with a tail?
Coma
Astigmatism
Chromatic aberration
Spherical aberration
What is the typical tube length for a microscope?
140 mm
150 mm
160 mm
180 mm
What type of lens creates a flat field of view?
Neofluar
Plano
Immersion
Apochromatic
Which component of the microscope is adjustable to focus and center light on the optical axis?
Stand
Condenser
Nosepiece
Eyepiece
How is total magnification calculated?
Objective magnification × eyepiece magnification
Objective magnification × tube length
Objective × eyepiece × (working tube length / normal tube length)
Eyepiece magnification × tube length
What is the function of immersion oil?
Reduce chromatic aberration
Improve resolution by reducing light refraction
Flatten the field of view
Adjust magnification
What type of aberration produces curved images from straight lines?
Spherical aberration
Astigmatism
Distortion
Field curvature
What does the term "W" indicate on a lens?
Water immersion
Wide-angle magnification
Weak magnification
Weighted focus
What is the primary disadvantage of simple microscopes?
Complex focusing system
Limited magnification
Chromatic aberration
Requires immersion oil
What is the main purpose of chromatic aberration correction?
Align multiple lenses
Focus different wavelengths equally
Reduce distortion
Improve eyepiece magnification
What is "Petzval field curvature"?
Magnification increase near the periphery
Unequal focus between the center and edges of an image creating fish eye lens view
Lens misalignment causing distortion
Chromatic aberration correction
Which invention improved the microscope to magnify up to 300x?
Compound lens system
Plano lens system
Single short-focus lens
Objective-based focusing
What does the eyepiece magnification typically equal?
4x
10x
40x
100x
What type of light behavior is used for magnification in simple microscopes?
Reflection
Diffraction
Refraction
Absorption
Which feature describes an apochromatic lens?
Immersion capability
Highly corrected for chromatic aberration
Wide-angle view
Low magnification
What results from a lens's inability to bring outer and central light rays to the same focus?
Chromatic aberration
Spherical aberration
Coma
Astigmatism
