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IB Bio Dna Replication Sl

Total questions: 23

Worksheet time: 23mins

Name
Class
Date
1.

What does a nucleosome consist of ?

a)

DNA and histones

b)

DNA and chromatin

c)

Chromatin and nucleotides

d)

Mature RNA and histones

2.

To which parts of the deoxyribose molecule do phosphates bind in DNA?

a)

I and V

b)

III and IV

c)

II and III

d)

III and V

3.

The drawing below shows a short section of a DNA molecule.


What is labelled by I, II and III?

a)

A

b)

B

c)

C

d)

D

4.

What are pyrimidines in DNA?

a)

Types of nucleotides

b)

Types of base pairs

c)

Types of sugars

d)

Types of bases

5.

What are Okazaki fragments?

a)

Sections of RNA removed by DNA polymerase III and replaced with DNA

b)

Nucleotides added by DNA polymerase I in the same direction as the replication fork

c)

Short sections of DNA formed during DNA replication

d)

Short lengths of RNA primase attached to the DNA during replication

6.

What is the reason for Okazaki fragments being formed during DNA replication?

a)

To enable replication of the 3′ → 5′ (lagging) strand

b)

To form the template for the RNA primers

c)

To initiate replication on the 5′ → 3′ (leading) strand

d)

To help the DNA helicase unwinding the DNA helix

7.

What happens during the formation of Okazaki fragments?

a)

DNA polymerase I adds nucleotides in the 5´ → 3´ direction.

b)

RNA polymerase adds nucleotides in the 3´ → 5´ direction.

c)

DNA polymerase III adds nucleotides in the 5´ → 3´ direction.

d)

DNA polymerase III adds nucleotides in the 3´ → 5´ direction.

8.

The diagram below shows part of a DNA molecule that is being replicated.


Where would DNA polymerase link the next nucleotide during replication?

a)

I

b)

II

c)

III

d)

IV

9.

Which enzyme removes the RNA primer during replication?

a)

RNA primase

b)

DNA polymerase I

c)

DNA helicase

d)

DNA polymerase III

10.

What is the function of DNA polymerase I?

a)

To add appropriate nucleotides in the 3′ --> 5′ direction

b)

To remove the RNA primers and replace them with DNA

c)

To join together the Okazaki fragments

d)

To join together both strands of DNA to the histones

11.

The diagram below represents DNA replication. Some of the bases are indicated.


In which direction is the replication fork moving and which bases would be needed to replicate the section of DNA shown?

a)

Direction = left to right; Bases = U, G and C

b)

Direction = right to left; Bases = U, G and C

c)

Direction = left to right; Bases = T, G and C

d)

Direction = right to left; Bases = T, G and C

12.

Where are free nucleotides added to a newly growing strand?

a)

to the 5'OH group of the previous deoxyribose sugar

b)

to the 5'OH group of the previous phosphate group

c)

to the 3'OH group of the previous deoxyribose sugar

d)

to the 3'OH group of the previous phosphate group

13.

Which is true of the lagging strand during DNA replication?

a)

DNA polymerase I adds nucleotides in a 5' --> 3' direction

b)

DNA polymerase III adds nucleotides in a 5' --> 3' direction

c)

DNA polymerase I adds nucleotides in a 3' --> 5' direction

d)

DNA polymerase III adds nucleotides in a 3' --> 5' direction

14.

What are Okazaki fragments made of?

a)

DNA only

b)

RNA only

c)

DNA and RNA

d)

Protein

15.

Why does a lagging strand need to be made during DNA replication?

a)

DNA polymerase I can only add nucleotides from 3' --> 5'

b)

DNA polymerase I can only add nucleotides from 5' --> 3'

c)

DNA polymerase III can only add nucleotides from 3' --> 5'

d)

DNA polymerase III can only add nucleotides from 5' --> 3'

16.

Which of the following is not true of the lagging strand during DNA replication?

a)

It is made from 5' --> 3'

b)

It is made in the same direction as the leading strand

c)

It is made by stopping and starting again

d)

RNA primers help it to be made

17.

What keeps the DNA strands apart during DNA replication?

a)

DNA gyrase

b)

DNA helicase

c)

DNA primase

d)

single-stranded binding proteins

18.

What is the role of RNA primers, which are needed to form the lagging strand?

a)

To allow RNA polymerase to bind

b)

To allow DNA polymerase to bind

c)

To allow DNA primase to bind

d)

To allow DNA ligase to bind

19.

How does DNA replicate?

a)

The deoxyribose of a free nucleotide is linked to the phosphate of the last nucleotide in the chain.

b)

The phosphate of a free nucleotide is linked to the deoxyribose of the last nucleotide in the chain.

c)

Nucleotides are linked in a 3′ to 5′ direction and the new strands are anti-parallel to the template strands.

d)

Nucleotides are linked in a 5′ to 3′ direction and the new strands are parallel to the template strands.

20.

What is the reason for Okazaki fragments being formed during DNA replication?

a)

To enable replication of the 3′ → 5′ (lagging) strand

b)

To form the template for the RNA primers

c)

To initiate replication on the 5′ → 3′ (leading) strand

d)

To help the DNA helicase unwinding the DNA helix

21.

What are the fundamental packaging units of eukaryotic chromosomes?

a)

Nucleosomes

b)

Centromeres

c)

Histones

d)

Nucleoids

22.

Which of the following is/are true of nucleosomes?


I. They contain six histone proteins

II. They control gene expression

III. They help in DNA supercoiling

a)

I only

b)

II only

c)

II and III only

d)

I, II and III

23.

How is a new nucleotide added to a growing DNA strand?

a)

The phosphate of the new nucleotide attaches to the 3'C of the previous nucleotide

b)

The phosphate of the new nucleotide attaches to the 5'C of the previous nucleotide

c)

The 3'C of the new nucleotide attaches to the phosphate of the previous nucleotide

d)

The 5'C of the new nucleotide attaches to the phosphate of the previous nucleotide