WorksheetsAseptic Technique and Performing Isolation Streaks
Total questions: 8
Worksheet time: 4mins
The use of sterile techniques and agar media enabled early microbiologists to:
Study pure cultures
Demonstrate the nature of infectious disease
Study mixed cultures
Study agar-metabolizing cultures e
What are the requirements for isolation?
Culture medium
Petri dish (agar plate)
Separation tools
All of the above
What is the first step in the streak plate method of isolation technique
Flame loop in between each quadrant that you make (cool before streaking)
Use the thin edge of the loop to give better isolation than the flat part
Hold the plate in the opposite hand while streaking (do not lay flat on the lab benchtop)
Keep plates closed between streaking (don't talk, cough on plates, etc)
What is the third step in the streak plate method of isolation technique
Flame loop in between each quadrant that you make (cool before streaking)
Use the thin edge of the loop to give better isolation than the flat part
Hold the plate in the opposite hand while streaking (do not lay flat on the lab benchtop)
Keep plates closed between streaking (don't talk, cough on plates, etc)
In what quadrant do the isolated colonies appear in the streak plate method of isolation?
1st quadrant
2nd quadrant
4th quadrant
All quadrant
A culture medium consisting of agar, human blood, and beef heart is a _____ medium.
Complex
Selective
Differential
Chemically defined
