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Enzyme Catalase

Total questions: 15

Worksheet time: 10mins

Name
Class
Date
1.
What is the primary role of enzymes inside of living cells? 
a)
organizing the translation of proteins
b)
allowing cells to be motile
c)
absorbing ultraviolet rays
d)
speeding up chemical reactions 
2.
Based on their role in the cell, enzymes can also be classified as –
a)
inhibitors
b)
catalysts
c)
 hormones
d)
vitamins
3.
Enzymes are a form of which biomolecule
a)
carbohydrates
b)
proteins
c)
nucleic acids
d)
lipids
4.
Enzymes are composed of which of the following monomers
a)
amino acids
b)
acyl groups
c)
nucleotides
d)
monosaccharides
5.
The enzyme catalase speeds up the chemical reaction that changes hydrogen peroxide into oxygen and water. The amount of oxygen given off is an indication of the rate of the reaction. Based on the graph, what can you conclude?
a)
Reaction rate decreases with increasing enzyme concentration.
b)
Reaction rate increases with decreasing enzyme concentration.
c)
Reaction rate increases with increasing enzyme concentration.
d)
The variables are indirectly proportional.
6.
Which of the factors does NOT affect enzymatic activity?
a)
Temperature
b)
pH
c)
Concentration
d)
Color
7.

What is the substrate in this enzyme substrate reaction?

a)

hydrogen (H2)

b)

hydrogen peroxide (H2O2)

c)

water (H2O)

d)

catalase

e)

oxygen (O2)

8.

The independent variable is what is altered in an experiment. What is the independent variable we are tested?

a)

different enzyme concentrations

b)

change the temperature

c)

different concentrations of hydrogen peroxide

d)

changing the temperature

9.

What is the name of the enzyme that speeds up the reaction in this lab

a)

catalase

b)

catalyst

c)

potato

d)

hydrogen peroxide (H2O2)

10.
What was the substrate in our enzyme lab?
a)
Hydrogen peroxide
b)
Catalase
c)
Yeast
d)
Oxygen gas
11.

As your mixture turned colors...

a)

The reaction progressed with the digestion of H2O2

b)

The reaction progressed with the synthesis of H2O2

c)

The catalase concentration increased

d)

The catalase concentration decreased

12.

If we changed the independent variable to temperature to study the affect it has on enzyme activity... which of the following components would we replace?

a)

H2O2

b)

Concentrations of catalase

c)

Guaiacol

d)

Buffers

13.

Why did you use guaiacol?

a)

It was the enzyme that directed the chemical reaction.

b)

It reacted with a product of the reaction to show the reaction taking place.

c)

It was the substrate of the reaction being broken down.

d)

It was used to neutralize free radicals to prevent the destruction of the enzyme.

14.

How did you measure the enzyme reaction taking place?

a)

The mixture changed color as the reaction took place.

b)

There was a temperature change as the reaction took place.

c)

Bubbles were produced as a result of H2O2 being broken down into water and oxygen.

d)

The pH of the mixture changed as the reaction took place.

15.

Why was there a Cup1 and Cup2?

a)

To keep the Guaiacol from reacting with the substrate until you were ready to record.

b)

To keep the substrate separate from the enzyme until you were ready to record.

c)

To keep the enzyme separate from the guaiacol until you were ready to record.

d)

To keep all of the reactants of the reaction from being contaminated prior to the start of the reaction.