Free Printable Thin Layer Chromatography

Worksheets

Thin Layer Chromatography

Total questions: 21

Worksheet time: 11mins

Name

Class

Date

TLC stands for...

Tender Love & Care

Thick Layer Chromatography

Thin Layer Chromatography

Thin Liquid Chromatography

The distance travelled by the compound depends on how well it adheres to the plate.

TRUE

FALSE

The distance travelled by the compound depends on how soluble it is in the chosen solvent.

TRUE

FALSE

How to calculate Rf?

Rf = distance travelled by sample / distance travelled by solvent

Rf = distance travelled by solvent / distance travelled by sample

When could a co-spot be used?

to make a direct comparison on a TLC plate between the tested compound and the pure substance

to improve the solubility of the tested compound in the solvent

to increase the Rf value

to decrease the Rf value

TLC can be used to_____. Tick all that apply

assess the purity of substances

confirm the identity of a compound

separate a mixture of immiscible liquids

purify a substance

_________________ utilizes a stationary and a mobile phase to separate mixtures based on their affinity for each phase.

filtration

chromatography

distillation

osmosis

The mobile phase in paper chromatography is typically a

plasma

solid

gas

liquid

The substance in the mixture that has the greatest affinity for the mobile phase will travel

fastest

slowest

10.

The substance in the mixture that has the greatest affinity for the stationary phase will travel

fastest

slowest

11.

The retention factor is used

to zero the chromatography scale

to identify unknown compounds in a sample

to retain solvent

as a starting tool for chromatography

12.

A qualitative test will reveal....

identity of a material

amount of a sample

usefulness of a compound

purity of a substance

13.

Which instrument is used to measure and record the absorption spectrum of a chemical substance?

Laser

TLC

Mass spectrograph

Spectrophotometer

14.

Which forms of Chromatography give qualitative and quantitative results?
I paper chromatoraphy
II TLC
III HPLC
IV GC

I and II

III and IV

II only

III only

15.

When using water as a solvent which of these molecules is likely to have the highest R_fvalue

ethanol

ethanoic acid

ethane

ethene

16.

In HPLC with a polar solvent which components are eluted first

large polar molecules

small non polar molecules

small polar molecules

large non polar molecules

17.

Which of the following can be used to find the concentration of a substance in HPLC

R_f value

R_t value

peak area

peak width

18.

In order to compare to chromatograms what must be kept the same?

stationary phase

mobile phase

temperature

all of the above

19.

Which of the following would give greater resolution in GC and HPLC

increased temperature

longer column

fast flow rate

increased pressure

20.

What two properties affect the R_t of a compound?
I Size/weight
II Polarity
III melting point

I only

II only

III only

I and II

21.

Which of the following factors would not affect an R_f value

solvent used

time taken

temperature

paper used

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Thin Layer Chromatography

Total questions: 21

Worksheet time: 11mins

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