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AP Biology Gene Regulation

Total questions: 15

Worksheet time: 15mins

Name
Class
Date
1.

What does the operon model attempt to explain?

a)

the coordinated control of gene expression in bacteria

b)

the mechanism of viral attachment to a host cell

2.

Transcription of the structural genes in an inducible operon

a)

occurs continuously in the cell.

b)

starts when the pathwayʹs substrate is present.

3.

Muscle cells and nerve cells in one species of animal owe their differences in structure to

a)

having different genes.

b)

having different genes expressed.

4.

If you were to observe the activity of methylated DNA, you would expect it to

a)

be unwinding in preparation for protein synthesis.

b)

have turned off or slowed down the process of transcription.

5.

Which enzyme was used to produce the molecule in the Figure?

a)

ligase

b)

a restriction enzyme

6.

Which of the following describe the structure of mature RNA? (more than 1 answer applies)

a)

Contains Uracil

b)

Contains introns and exons

c)

Has a poly-A tail and 5' cap

d)

Single stranded

e)

Circular strand

7.

Which of the following covalently connects segments of DNA?

a)

helicase

b)

DNA polymerase

c)

ligase

8.

Based your answer on the figure: A mutation results in a defective enzyme A. Which of the following would be a consequence of that mutation?

a)

an accumulation of A and no production of B and C

b)

an accumulation of A and B and no production of C

9.

What is the enzymatic function of restriction enzymes?

a)

to cleave nucleic acids at specific sites

b)

to add new nucleotides to the growing strand of DNA

10.

Which enzyme catalyzes the elongation of a DNA strand in the 5ʹ → 3ʹ direction?

a)

primase

b)

DNA ligase

c)

DNA Polymerase

d)

helicase

11.

In the lac operon of E. coli, what is the role of the repressor protein?

a)

A) It binds to the operator to prevent transcription.

b)

B) It enhances the binding of RNA polymerase to the promoter.

c)

C) It acts as a co-repressor to activate transcription.

d)

D) It degrades lactose to glucose and galactose.

12.

Which of the following best describes the function of an enhancer in eukaryotic gene regulation?

a)

A) It directly binds to RNA polymerase to initiate transcription.

b)

B) It increases the rate of transcription by binding to transcription factors.

c)

C) It silences gene expression by binding to repressor proteins.

d)

D) It is a sequence that is transcribed into mRNA.

13.

What is the primary function of the TATA box in eukaryotic promoters?

a)

A) It signals the termination of transcription.

b)

B) It serves as a binding site for ribosomes during translation.

c)

C) It is the recognition site for the binding of transcription factors.

d)

D) It is involved in the splicing of introns.

14.

In an experiment, a researcher adds a molecule that mimics the substrate of an inducible operon. What is the expected outcome?

a)

A) The operon will be repressed, and transcription will decrease.

b)

B) The operon will be induced, and transcription will increase.

c)

C) The operon will be unaffected, and transcription will remain constant.

d)

D) The operon will be permanently turned off.

15.

How does DNA methylation affect gene expression in eukaryotic cells?

a)

A) It activates gene expression by loosening chromatin structure.

b)

B) It represses gene expression by tightening chromatin structure.

c)

C) It has no effect on gene expression.

d)

D) It enhances gene expression by recruiting RNA polymerase.