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WorksheetsPlasmid
Total questions: 15
Worksheet time: 15mins
Scientists have taken advantage of __________ to use them as tools to clone, transfer, and manipulate genes by inserting genes from other species to create new genetic combinations.
DNA fingerprints
plasmids
GMOs
genomes
Circular shaped DNA found in bacteria (a)
plasmid
DNA fingerprinting
biotechnology
vector
GMO
nucleus
All plasmids have a promoter region which is necessary for
DNA to be duplicated
DNA to be transcribed into RNA
DNA to be inserted easily
DNA to be cut open
What occurs during transformation?
The use of viruses to transform or genetically
engineer cells.
One bacterium passes a plasmid to another through a pilus
The insertion of a foreign plasmid into a bacterial cell which results in new acquired traits.
Genetic engineering or transformation of mammalian cells.
Observe the results above. What gene is present in the engineered plasmid?
kanamycin resistant gene
ampicillin resistant gene
tetracycline resistant gene
No plasmid is necessary to grow on plate 3.
Examine the results for group 4. Why would this group see growth without RFP in the third plate?
The bacteria cells did not transform.
The bacteria cells transformed, but did not incorporate the plasmid containing the RFP gene.
The bacteria cells transformed, but did not incorporate the plasmid with the arabinose gene.
Group 4 did not observe growth in the third plate.
Which one of these agar plates would there be no bacteria growth?
+pGLO LB/amp
+pGLO LB/amp/ara
-pGLO LB/amp
-pGLO LB
Ampicillin (antibiotic) is used in the plates to
isolate and select for only the bacteria that have been successfully transformed
give bacteria energy to grow
act as a trigger to turn on the expression of the gene of interest
give the bacteria a new trait (such as fluorescence or insulin production)
Why is calcium chloride used to make bacterial cells more competent during transformation?
The cations from CaCl2 allow for the negative plasmid to get close to the negative plasma membrane
The ice cold CaCl2 protects the cells from denaturing during heat shock
The anions from CaCl2 allows the positive plasmid to get close to the positive plasma membrane
Ice cold CaCl2 helps close membrane pores so that the plasmid cannot leave the cell
Which of the following is a common use of plasmids in genetic engineering?
Gene cloning
Protein synthesis
Photosynthesis
Cell respiration
What is the primary role of a plasmid in bacterial cells?
Energy production
Genetic replication
Antibiotic resistance
Photosynthesis
In a laboratory setting, how are plasmids typically introduced into bacterial cells?
Conjugation
Transformation
Transduction
Binary fission
Which feature of plasmids makes them useful for gene cloning?
Their ability to replicate independently
Their large size
Their ability to produce energy
Their role in photosynthesis
What is a selectable marker in the context of plasmids?
A gene that allows for the identification of bacteria that have taken up the plasmid
A gene that causes the plasmid to replicate
A gene that makes the plasmid visible under a microscope
A gene that allows the plasmid to photosynthesize
How do plasmids contribute to antibiotic resistance in bacteria?
By producing antibiotics
By carrying genes that confer resistance
By destroying antibiotics
By preventing antibiotic entry into the cell
