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Worksheets

MI 3.4

Total questions: 25

Worksheet time: 25mins

Name
Class
Date
1.

A microscope ______________________objects.

a)

condenses

b)

magnifies

c)

eliminates

d)

electrifies

2.

Which microbe is the image most likely to be?

a)

Bacteria

b)

Fungus

c)

Virus

d)

None of the above

3.

What do viruses need to reproduce?

a)

they need genetic material

b)

They need a host cell

c)

They need  bacteria

d)

They need insulin

4.

Select the correct definition to the correct microbes

a)

Virus

1.

Contains DNA and a protein coat

b)

Bacteria

2.

No Nucleus and Unicellular

c)

Protist

3.

Has a nucleus, unicellular or multicellular, autotroph or heterotroph

d)

Fungi

4.

Has a nucleus, mainly multicellular, and heterotrophs

5.

List the steps of the scientific method

6.

Reorder the microbe groups from simplest to most complex

a)

Archaea

b)

Bacteria

c)

Protists

d)

Fungi

1)
2)
3)
4)
7.

Which microbe is the image most likely to be?

a)

Bacteria

b)

Fungus

c)

Virus

d)

None of the above

8.

Bacteria reproduce asexually and result in offspring that are

a)

identical

b)

different

9.

Which of the following is a true statement about bacteria?

a)

Bacteria exist in only a few environments on earth

b)

Bacteria can grow to extremely large sizes

c)

Most bacteria are harmful to humans

d)

Bacteria can be both harmful and helpful to humans

10.

Reasons that viruses are considered to be nonliving is because

a)

They are not cellular

b)

They cannot reproduce on their own

c)

They cannot make energy

d)

all of the above

11.

A virus contains ______, the genetic material it uses to invade a living thing.

a)

DNA

b)

RNA

c)

ATP

d)

Cells

12.

During PCR, what happens during the Denaturation step?

a)

DNA strands are separated according to size using gel electrophoresis.

b)

Primers form base pairs with the single-stranded template DNA.

c)

DNA polymerase adds new nucleotides to the primer to elongate the new strand.

d)

Hydrogen bonds between bases are broken using high heat to separate DNA strands.

13.

Which of the following statements are true about DNA structure?

a)

The DNA backbone ("sides of the ladder") is made up of sugars and phosphates.

b)

The sugars and phosphates make up the unique code of each gene.

c)

The DNA backbone is the same for every person.

d)

Bases and sugars pair together to form the "rungs" of the DNA ladder.

e)

The sequence of bases is unique for each person.

14.

During which PCR step do primers form complementary base pairs with single-stranded template DNA?

a)

Denaturation

b)

Annealing

c)

Extension

d)

Synthesis

15.

Which part of Sanger (Cycle) Sequencing is NOT found in regular Polymerase Chain Reaction (PCR)?

a)

DNA polymerase

b)

Primers

c)

Deoxyribonucleotides (dNTPs)

d)

Dideoxyribonucleotides (ddNTPs)

e)

DNA template

16.

How are deoxyribonucleotides different from dideoxyribonucleotides? Choose all that apply.

a)

Deoxyribonucleotides are used in both PCR and Cycle Sequencing.

b)

Dideoxyribonucleotides are missing an OH group compared to deoxyribonucleotides.

c)

You cannot add new nucleotides to a deoxyribonucleotide.

d)

Deoxyribonucleotides are NOT found in natural living cells.

e)

Dideoxyribonucleotides are labeled with fluorescent tags.

17.

How is DNA synthesis in PCR and Cycle Sequencing the SAME?

a)

Both require DNA polymerase.

b)

Both require primers.

c)

Both require deoxynucleotides (dNTPs).

d)

Both require dideoxynucleotides (dNTPs).

e)

Both generate new DNA products of equal length.

18.

What is the primary function of a microscope?

a)

To condense objects

b)

To magnify objects

c)

To eliminate objects

d)

To electrify objects

19.

What do viruses need to reproduce?

a)

Genetic material

b)

A host cell

c)

Bacteria

d)

Insulin

20.

Select the correct definition for microbes.

a)

Virus

b)

Bacteria

c)

Protist

d)

Fungi

21.

Reorder the microbe groups from simplest to most complex.

a)

Archaea

b)

Bacteria

c)

Protists

d)

Fungi

22.

Bacteria reproduce asexually and result in offspring that are:

a)

Identical

b)

Different

23.

Which of the following is a true statement about bacteria?

a)

Bacteria exist in only a few environments on earth

b)

Bacteria can grow to extremely large sizes

c)

Most bacteria are harmful to humans

d)

Bacteria can be both harmful and helpful to humans

24.

Reasons that viruses are considered to be nonliving include:

a)

They are not cellular

b)

They cannot reproduce on their own

c)

They cannot make energy

d)

All of the above

25.

A virus contains ______, the genetic material it uses to invade a living thing.

a)

DNA

b)

RNA

c)

ATP

d)

Cells