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Molecular Diagnostics, Biosensor Development, and Microfludics

Total questions: 10

Worksheet time: 32mins

Name
Class
Date
1.

How can you increase the sensitivity in molecular diagnostics?

4 lines
2.

Intercalating dyes bind to single stranded DNA.

a)

True

b)

False

3.

Short single stranded DNA sequences that recognise conserved regions in an organism's nucleic acid are called?

4 lines
4.

What is PCR?

4 lines
5.

What are the 3 stages in PCR and what conditions are required for them?

4 lines
6.

Dyes which aid the visualization of DNA amplification are called?

4 lines
7.

Apart from dyes, how can you determine the success of nucleic acid amplification?

4 lines
8.

Which enzyme is responsible for DNA amplification?

4 lines
9.

I am interested in detecting the virus responsible for Dengue hemorrhagic fever. It is an RNA virus. Describe the steps l need to detect this virus using molecular diagnostic principles.

4 lines
10.

What are the steps involved in DNA extraction?

4 lines